We narrowed to 1,639 results for: plasmid for e coli

1. pET11a_His-TEV-PLpro (nsp3) (SARS-CoV-2)

   Plasmid

   #169192

   Purpose

   To express SARS-CoV-2 Papain-like protease in E. coli

   Depositor

   John Diffley

   Article

   Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

   Insert

   6His-TEV-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Unspecified

   Tags

   6His-TEV

   Expression

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEC3079 (p7INT-Pveg_lacI)

   Plasmid

   #218505

   Purpose

   integrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   lacI (lacI Escherichia coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pveg

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPLtetO-FtsA

   Plasmid

   #107558

   Purpose

   Plasmid for titrating synthesis of FtsA with doxycycline

   Depositor

   Uwe Sauer

   Article

   Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

   Insert

   ftsA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pLtetO

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPLtetO-FtsZ

   Plasmid

   #89656

   Purpose

   Plasmid for titrating synthesis of FtsZ with doxycycline

   Depositor

   Uwe Sauer

   Article

   Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

   Insert

   GTP-binding tubulin-like cell division protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHC01

   Plasmid

   #106900

   Purpose

   Ratiometric gas biosensor plasmid for 3-oxo-C12-HSL sensing

   Depositor

   Jonathan Silberg

   Article

   Cheng et al ACS Synth Biol. 2018 Jan 24. doi: 10.102

   Inserts

   LasR
   Methyl Halide Transferase
   Ethylene Forming Enzyme
   Red Fluorescent Protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJM149

   Plasmid

   #134168

   Purpose

   Retroviral plasmid expressing GFP - Nde1

   Depositor

   Iain Cheeseman

   Article

   Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

   Insert

   Nde1_SSSC E118A R129A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SSSC E118A R129A, E. coli codon optimized, CRISPR…

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJM147

   Plasmid

   #134167

   Purpose

   Retroviral plasmid expressing GFP - Nde1

   Depositor

   Iain Cheeseman

   Article

   Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

   Insert

   Nde1_SSSC E47A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SSSC E47A, E. coli codon optimized, CRISPR/Cas9 h…

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJ02B2Gm_EF

   Plasmid

   #66064

   Purpose

   MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   FACS Controls
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJM143

   Plasmid

   #134166

   Purpose

   Retroviral plasmid expressing GFP - Nde1

   Depositor

   Iain Cheeseman

   Article

   Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

   Insert

   Nde1_SSSC 1-220 E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SSSC, E. coli codon optimized, CRISPR/Cas9 harden…

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPLtetO-PdhR

    Plasmid

    #89655

    Purpose

    Plasmid for titrating synthesis of PdhR with doxycycline

    Depositor

    Uwe Sauer

    Article

    Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

    Insert

    Pyruvate dehydrogenase complex repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPLtetO-FtsB

    Plasmid

    #107559

    Purpose

    Plasmid for titrating synthesis of FtsB with doxycycline

    Depositor

    Uwe Sauer

    Article

    Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

    Insert

    ftsB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLtetO

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJ02B2Rm_EF

    Plasmid

    #66060

    Purpose

    MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    FACS Controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJ02B2Rm_EF

    Plasmid

    #66066

    Purpose

    MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    FACS Controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJ02B2Gm_EF

    Plasmid

    #66058

    Purpose

    MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    FACS Controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pARC8-LambdaRedBeta-EcSSB

    Plasmid

    #162572

    Purpose

    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency.

    Depositor

    George Church

    Article

    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

    Inserts

    Lambda Red-Beta
    E. coli SSB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    May 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA

    Plasmid

    #228787

    Purpose

    EcTyr aaRS/tRNA system for TAG suppression with EGFP containing three stop codons for triple suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTyrRS
    EcYtR
    EGFP***
    TEV protease
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA

    Plasmid

    #228788

    Purpose

    EcTyr aaRS/tRNA system for pAAF incorporation with EGFP containing three stop codons for triple suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTyrRS
    EcYtR
    EGFP***
    TEV protease
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAcBac3-EcTrp-TGA-EGFP**

    Plasmid

    #228786

    Purpose

    EcTrp aaRS/tRNA system for TGA suppression with EGFP containing two stop codons for dual suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTrpRS
    EcWtR
    EGFP**
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TGA suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCJ136

    Plasmid

    #162665

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCJ207

    Plasmid

    #162680

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge