We narrowed to 9,413 results for: try

1. mCherry-eDHFR-Cdc42Q61L

   Plasmid

   #107267

   Purpose

   mCherry-eDHFR-Cdc42Q61L in pIVT vector for in vitro transcription. Note that Cdc42 in this plasmid keeps its CAAX domain.

   Depositor

   Michael Lampson

   Article

   Akera et al Science. 2017 Nov 3;358(6363):668-672. d

   Insert

   mCherry-eDHFR-Cdc42Q61L (CDC42, dhfr Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

   Plasmid

   #121817

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ289 (ErCas12a)

   Plasmid

   #138126

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   ErCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 139H2_HC

   Plasmid

   #206201

   Purpose

   For recombinant expression of the full heavy chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells. Includes a C-terminal -His8 tag for purification.

   Depositor

   Joost Snijder

   Article

   Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

   Insert

   anti-MUC1 antibody 139H2 heavy chain (Ighg1 Mouse)
   

   Use

   Tags

   -AAAHHHHHHHH

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

   Plasmid

   #121814

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNUT N6His K206E/K534A hTFNG

   Plasmid

   #70133

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobe

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Halbrooks et al Biochemistry. 2003 Apr 8;42(13):3701-7.

   Insert

   N6His K206e/K534A hTFNG (TF Human)
   

   Use

   Tags

   N-terminal signal peptide, 4 aa link, 6 His, Fact…

   Expression

   Mammalian

   Mutation

   changed Lys206 to a glutamic acid and Lys534 to a…

   Promoter

   SV40

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-2B-Gata4

   Plasmid

   #98615

   Purpose

   Gateway entry vector for Gata4

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   Gata4 (Gata4 Mouse)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKK-NoTag

   Plasmid

   #105767

   Purpose

   Control vector for pKK series (encodes only SLIC arms (TEV-L and TEV-R)); expression without tag. Useful to design other vectors; any tag can be added.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

   Plasmid

   #121841

   Purpose

   pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-hPMCA2xb

    Plasmid

    #47751

    Purpose

    mammalian expression of mCherry tagged hPMCA2, xb splice variant

    Depositor

    Emanuel Strehler

    Article

    Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi

    Insert

    PMCA2x/b (ATP2B2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    xb splice variant

    Promoter

    CMV

    Available since

    Nov. 23, 2018

    Availability

    Academic Institutions and Nonprofits only