We narrowed to 114,361 results for: MPI

1. Phage-PGK-FLAG-HA-T(WT)

   Plasmid

   #181737

   Purpose

   for lentiviral expression of brachyury ORF in mammalian cells

   Depositor

   Charles Lin

   Insert

   T (brachyury)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   a silent mutation in the PAM site of an sgRNAt ta…

   Promoter

   Available since

   Aug. 3, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pBCN43-R4R3

   Plasmid

   #34918

   Depositor

   Ben Lehner

   Article

   Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAM-35s-Lyk5-YFPn-1

   Plasmid

   #102389

   Purpose

   split YFP. Plant expression of Lyk5-YFPn-1

   Depositor

   Silke Robatzek

   Insert

   LYK5 (LYK5 Mustard Weed)
   

   Use

   Tags

   YFPn

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX4T1-WWP1F792D

   Plasmid

   #179328

   Purpose

   E. coli optimized expression of GST-fusion of human WWP1 F792D

   Depositor

   Arthur Haas

   Insert

   WWP1 F792D (WWP1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F792D mutation; Codon Optimized for expression in…

   Promoter

   Available since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBtet-RB-PE2

   Plasmid

   #173903

   Purpose

   SB-transposon with inducible expression of SpCas9 PE2

   Depositor

   Ronald Cohn

   Insert

   PE2
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. XE183 rat Frizzled-2-pCD

   Plasmid

   #16813

   Depositor

   Randall Moon

   Insert

   frizzled-2 (Fzd2 Rat)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA5/FRT/TO/Flag-mKO2

   Plasmid

   #110371

   Purpose

   Mammalian expression of mKO2 with FLAG tag, Flp-In T-Rex system

   Depositor

   Sandra Martha Gomes Dias

   Insert

   monomeric Kusabira Orange 2
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPS2972

   Plasmid

   #8942

   Depositor

   Pamela Silver

   Article

   Devroe et al BMC Biotechnol. 2002 Aug 28. 2():15.

   Insert

   NDR1 (STK38 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

9. TC9nickpAB

   Plasmid

   #71311

   Purpose

   used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI

   Depositor

   Karl Degenhardt

   Insert

   Cas9 nickase
   

   Use

   In vitro transcription

   Tags

   NLS

   Expression

   Mutation

   D10A nickase

   Promoter

   T7

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. LCMV:GFP(FRT)MCS

    Plasmid

    #31298

    Depositor

    Gerhart Ryffel

    Article

    Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

    Insert

    GFP(FRT)MCS
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIC65

    Plasmid

    #63061

    Purpose

    Retroviral expression of EGFP-TEV-S-tag Mis12 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    hMis12, IMAGE clone 3351245 (MIS12 Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Mutation

    L68R

    Promoter

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PPT1

    Plasmid

    #25205

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PPT1_02 (PPT1 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 12, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pnEA-NpH-HsBTG2_1-128opt_AF

    Plasmid

    #148656

    Purpose

    Bacterial Expression of HsBTG2_1-128opt

    Depositor

    Elisa Izaurralde

    Insert

    HsBTG2_1-128opt (BTG2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK406

    Plasmid

    #72420

    Purpose

    Produces Acetobacter aceti 1023 Est05, C-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    hypothetical protein
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    additional N-terminal MTFLYSICHIVMNIVSSTPHFNVWICF…

    Promoter

    T7

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1XDR4

    Plasmid

    #32614

    Depositor

    Connie Cepko

    Article

    Billings et al PLoS One. 2010 . 5(10):e13739.

    Insert

    1XDR4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNC820

    Plasmid

    #20383

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    UAS cassette (FUS1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    X=UbiM (see schematic)

    Promoter

    Available since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCN-ACO2

    Plasmid

    #45329

    Depositor

    Brendan Cormack

    Article

    Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

    Type

    Empty backbone

    Use

    C. glabrata expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS L30 mRuby3-Rab5c

    Plasmid

    #166426

    Purpose

    Rab5C fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Insert

    Rab5C
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    L30 weak promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMIY-Arg1-Flag

    Plasmid

    #34581

    Depositor

    Peter Murray

    Insert

    Arg1 (Arg1 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

20. CMV-d2eGFP-20

    Plasmid

    #21970

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    CMV d2eGFP miR-20 sponge
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only