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pVG1

Plasmid

#111444

Purpose

Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgScADE2/stop-codon repair

Use

CRISPR

Expression

Yeast

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV Syn ChR E90R-T159C 2A tDimer

Plasmid

#52495

Purpose

Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

Depositor

Thomas Oertner

Article

Wietek et al Science. 2014 Mar 27.

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Expression

Mammalian

Mutation

E90R, T159C

Promoter

human synapsin-1 and ribosomal skip sequence

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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pV1464

Plasmid

#111440

Purpose

N. castellii Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-Trib3

Plasmid

#131606

Purpose

expression of mouse Trib3 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Trib3 (Trib3 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcdna3 T7 Jip1b

Plasmid

#52122

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Tags

T7

Expression

Mammalian

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only
pCSC-ASCL1-IRES-Sox11

Plasmid

#182234

Purpose

To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189

Depositor

Chun-Li Zhang

Article

Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

Insert

ASCL1-IRES-Sox11

Use

Lentiviral

Promoter

CMV

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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UUUUA-pHG165c

Plasmid

#170107

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the UreR urea ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

UUUUA

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MMMMA-pHG165c

Plasmid

#170108

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the MelR L-melibiose ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

MelR

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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XXXXA-pHG165c

Plasmid

#170110

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

XXXXA

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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XXXXA_Q182A_R287H-pHG165c

Plasmid

#170111

Purpose

Constitutively expressed , mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

XXXXA_Q182A_R278H

Expression

Bacterial

Mutation

Q182A, R287H

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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RRRAA-pHG165c

Plasmid

#170112

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

RRRAA

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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LLhE_AW-pHG165

Plasmid

#90062

Purpose

Constitutive expression of a chimeric LacI:CelR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhE_AW

Expression

Bacterial

Mutation

I48V/Q55A/Q60R

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhS-pHG165a

Plasmid

#90040

Purpose

Constitutive expression of a chimeric LacI:GalS transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhS

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhF-pHG165a

Plasmid

#90041

Purpose

Constitutive expression of a chimeric LacI:FruR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhF

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhT-pHG165a

Plasmid

#90042

Purpose

Constitutive expression of a chimeric LacI:TreR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhT

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhR-pHG165a

Plasmid

#90043

Purpose

Constitutive expression of a chimeric LacI:RbsR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhR

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhA-pHG165a

Plasmid

#90044

Purpose

Constitutive expression of a chimeric LacI:AscG transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhA

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhE-pHG165

Plasmid

#90045

Purpose

Constitutive expression of a chimeric LacI:CelR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhE

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhC-pHG165a

Plasmid

#90046

Purpose

Constitutive expression of a chimeric LacI:CytR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhC

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcdna3 EE Jip1b

Plasmid

#52127

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Tags

EE

Expression

Mammalian

Available Since

April 18, 2014

Availability

Academic Institutions and Nonprofits only