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We narrowed to 7,609 results for: Pol

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Showing: 7601 - 7609 of 7609 results

  1. TfCut2_ICCG_b0.45_h80_dd90_02

    Plasmid
    #257186
    Purpose
    Expression construct for TfCut2_ICCG_b0.45_h80_dd90_02 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    TfCut2_ICCG_b0.45_h80_dd90_02
    Expression
    Bacterial
    Mutation
    R18T, S19R, E27T, N28S, A34V, R46T, E47T, N48T, N…
    Available Since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257186-plasmid-map-sequence-id-517229

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  2. Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)

    Plasmid
    #217797
    Purpose
    Variant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Cdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Cdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217797-plasmid-map-sequence-id-432864

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  3. pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter

    Plasmid
    #172599
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.
    Depositor
    Daniel Kiss
    Article
    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2
    Insert
    myc-PDGFRA-D842V+R914W (PDGFRA Human, Synthetic)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-D842V+R914W
    Promoter
    CMV
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172599-plasmid-map-sequence-id-338914

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  4. pCEP4-myc-PDGFRA-K627R+R914W_EGFP-PEST_reporter

    Plasmid
    #172600
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R+R914W mutant signaling.
    Depositor
    Daniel Kiss
    Article
    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2
    Insert
    myc-PDGFRA-K627R+R914W (PDGFRA Human, Synthetic)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-K627R+R914W
    Promoter
    CMV
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172600-plasmid-map-sequence-id-338836

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  5. pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

    Plasmid
    #131255
    Purpose
    Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131255-plasmid-map-sequence-id-267709

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  6. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid
    #131256
    Purpose
    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Contains point mutations that convert USP7 aspart…
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131256-plasmid-map-sequence-id-267711

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  7. CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

    Plasmid
    #91820
    Purpose
    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD
    Depositor
    Chris Hill
    Article
    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1
    Insert
    RPO21 (RPO21 Budding Yeast)
    Tags
    10xHis
    Expression
    Yeast
    Mutation
    5xGly linker, PreScission Protease site (LEVLFQGP…
    Promoter
    Endogenous Rpb1 promoter
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    91820-plasmid-map-sequence-id-181105

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  8. RLC-ELP-ybbR-LA-GB1-GB1-Xmod-DocIII-Ctag

    Plasmid
    #257334
    Purpose
    Polyprotein for applying force to the N- and C-termini of the lever arm in the RLC complex of human β-cardiac myosin
    Depositor
    Tom Perkins
    Article
    Deopa et al ACS Nano. 2026 Jun 9;20(22):15982-16000.
    Inserts
    RLC (MYL2 Human)
    Lever Arm (MYH7 Human)
    Tags
    Ctag, Dockerin III, ELP120 (Elastin-Like Polypept…
    Expression
    Bacterial
    Mutation
    only amino acids # 810-846 are used
    Promoter
    T7
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    257334-plasmid-map-sequence-id-512397

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  9. LA-ELP-ybbR-RLC-ddfln4-Xmod-DocIII-Ctag

    Plasmid
    #257335
    Purpose
    Polyprotein for applying force to the N- and C-termini of the RLC in the RLC complex of human β-cardiac myosin
    Depositor
    Tom Perkins
    Article
    Deopa et al ACS Nano. 2026 Jun 9;20(22):15982-16000.
    Inserts
    RLC (MYL2 Human)
    Lever Arm (MYH7 Human)
    Tags
    Ctag, Dockerin III, ELP120 (Elastin-Like Polypept…
    Expression
    Mammalian
    Mutation
    only amino acids # 810-846 are used
    Promoter
    T7
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    257335-plasmid-map-sequence-id-515911

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Showing: 7601 - 7609 of 7609 results