12,469 results

1. pKLV2-U6gRNA5(Rictor-g2)-PGKpuroBFP-W

   Plasmid

   #105042

   Purpose

   Lentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Rictor (Rictor Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKLV2-U6gRNA5(Zfp219-g1)-PGKpuroBFP-W

   Plasmid

   #105021

   Purpose

   Lentiviral gRNA plasmid targeting mouse Zfp219 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Zfp219 (Zfp219 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKLV2-U6gRNA5(Apc-g1)-PGKpuroBFP-W

   Plasmid

   #105022

   Purpose

   Lentiviral gRNA plasmid targeting mouse Apc , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Apc (Apc Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKLV2-U6gRNA5(Slc25a51-g1)-PGKpuroBFP-W

   Plasmid

   #105028

   Purpose

   Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Slc25a51 (Slc25a51 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKLV2-U6gRNA5(Slc25a51-g2)-PGKpuroBFP-W

   Plasmid

   #105029

   Purpose

   Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Slc25a51 (Slc25a51 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKLV2-U6gRNA5(Ndufa2-g1)-PGKpuroBFP-W

   Plasmid

   #105030

   Purpose

   Lentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Ndufa2 (Ndufa2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV2-U6gRNA5(Ndufa2-g2)-PGKpuroBFP-W

   Plasmid

   #105031

   Purpose

   Lentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Ndufa2 (Ndufa2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKLV2-U6gRNA5(Hgs-g1)-PGKpuroBFP-W

   Plasmid

   #105032

   Purpose

   Lentiviral gRNA plasmid targeting mouse Hgs , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   gRNA targeting Hgs (Hgs Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKLV2-U6gRNA5(Vps25-g1)-PGKpuroBFP-W

   Plasmid

   #105033

   Purpose

   Lentiviral gRNA plasmid targeting mouse Vps25 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Vps25 (Vps25 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKLV2-U6gRNA5(Pou5f1-g1)-PGKpuroBFP-W

    Plasmid

    #105035

    Purpose

    Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Pou5f1 (Pou5f1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKLV2-U6gRNA5(Tcf7l1-g1)-PGKpuroBFP-W

    Plasmid

    #105015

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    gRNA targeting Tcf7l1 (Tcf7l1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKLV2-U6gRNA5(Tcf7l1-g2)-PGKpuroBFP-W

    Plasmid

    #105016

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tcf7l1 (Tcf7l1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKLV2-U6gRNA5(Kcmf1-g1)-PGKpuroBFP-W

    Plasmid

    #105017

    Purpose

    Lentiviral gRNA plasmid targeting mouse Kcmf1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Kcmf1 (Kcmf1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKLV2-U6gRNA5(Pggt1b-g1)-PGKpuroBFP-W

    Plasmid

    #105018

    Purpose

    Lentiviral gRNA plasmid targeting mouse Pggt1b , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Pggt1b (Pggt1b Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKLV2-U6gRNA5(Znrf1-g1)-PGKpuroBFP-W

    Plasmid

    #105020

    Purpose

    Lentiviral gRNA plasmid targeting mouse Znrf1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Znrf1 (Znrf1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3 T2P 500

    Plasmid

    #131150

    Purpose

    To investigate activity of a 500 bp region upstream of mouse Trib2.

    Depositor

    Karen Keeshan

    Article

    Rishi et al Blood. 2014 Apr 10;123(15):2389-400. doi

    Insert

    -500 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
    

    Use

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDM304_YFP-IqgC_mRFP-Raf1(RBD)

    Plasmid

    #128698

    Purpose

    simultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cells

    Depositor

    Igor Weber

    Article

    Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;11

    Inserts

    iqgC (full length)
    Raf1 kinase (aa 50-132)
    

    Use

    Dictyostelium discoideum expression vector

    Tags

    YFP and mRFP

    Expression

    Mutation

    none

    Promoter

    Available since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28B-PU.1-ETS-H3

    Plasmid

    #124435

    Purpose

    Chimeric ETS domain: H3 of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Residues 225 to 239 replaced with corresponding s…

    Promoter

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. IgH P+ VA20

    Plasmid

    #127249

    Purpose

    IgH V-region

    Depositor

    Christine Milcarek

    Article

    Shell et al J Immunol. 2007 Dec 1;179(11):7663-73.

    Insert

    Promoter+V-region of IgH of A20 B cell line
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    IgH

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Human/mouse ELL2

    Plasmid

    #127254

    Purpose

    Expresses a human/mouse chimera that increases ELL2 expression in mammalian cells

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    N-terminal human/C-terminal mouse ELL2 (ELL2, Ell2 Mouse, Human)
    

    Use

    Other

    Tags

    His6X

    Expression

    Mammalian

    Mutation

    N terminal amino acids (1-287) are human, C termi…

    Promoter

    pEF1a

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ELL3 Streptag CtoA

    Plasmid

    #127283

    Purpose

    Expresses mouse ELL3 with streptactin tag; To interrogate if the mutation decreases putative Myc activity

    Depositor

    Christine Milcarek

    Insert

    ELL3 (NM_145973) (ELL3 Mouse)
    

    Use

    Tags

    Twin strep-tag

    Expression

    Mammalian

    Mutation

    CEYLHNK to AEYLHQK

    Promoter

    EF1a

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MSCV-Lrrc40-trunc-IRES-BFP

    Plasmid

    #117268

    Purpose

    Retroviral overexpression of Lrrc40 - truncated version with seemingly same DE genes in Th2 as MSCV-Lrrc40-IRES-BFP

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    Lrrc40 (Lrrc40 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    The insert is truncated - deletion of bp 334-407 …

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-hSyn-NOS-IN133.3xFLAG-WPRE

    Plasmid

    #127869

    Purpose

    pAAV plasmid expressing an NOS-IN133.3xFLAG fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1 (Nos1 Mouse)
    

    Use

    AAV

    Tags

    3xFLAG

    Expression

    Mutation

    Amino acids 1-133 only

    Promoter

    hSyn

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA-FLAG-Intu

    Plasmid

    #128869

    Purpose

    For mammalian expression of FLAG-tagged mouse Inturned

    Depositor

    Aimin Liu

    Insert

    Inturned (Intu Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128347

    Purpose

    pAAV encoding control gRNA for CRISPR gRNAs listed above

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    control (negative)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128345

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrnb4 (Chrnb4 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFB-U6-Chrna6-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128341

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna6 (Chrna6 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ELL1 Streptag

    Plasmid

    #127278

    Purpose

    Expresses mouse ELL1 with streptactin tag in mammalian cells

    Depositor

    Christine Milcarek

    Insert

    ELL1 (NM_007924) (ELL Mouse)
    

    Use

    Tags

    Twin strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Myc var1 K397R

    Plasmid

    #127285

    Purpose

    Expresses Myc with putative Ub site mutated

    Depositor

    Christine Milcarek

    Insert

    Myc (NM_001177352) (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K397R by Q5 mutagenesis

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Myc var1

    Plasmid

    #127284

    Purpose

    Expresses Myc to see if degraded by ELLs

    Depositor

    Christine Milcarek

    Insert

    Myc (NM_001177352) (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    transcript variant 1

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge