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pSL21-mCherry

Plasmid

#164410

Purpose

Retroviral expression plasmid of sgRNA with mCherry

Depositor

Junwei Shi

Article

Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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FlucDM-HA-GFP11-N1

Plasmid

#91956

Purpose

Expresses aggregation-prone FlucDM-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucDM-HA-GFP11

Expression

Mammalian

Mutation

R188Q,R261Q

Promoter

CMV

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pBEVY-L

Plasmid

#51226

Purpose

bi-directional expression vector for yeast, constitutively active, LEU2 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GPD/ADH1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCMMP-MCS-IRES-Puro

Plasmid

#36952

Depositor

Bill Sugden

Article

Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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FUW-KM

Plasmid

#20332

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Klf4-F2A-cMyc

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pPolII-Luc

Plasmid

#17223

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

PolII promoter

Use

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pAAV.GFAP.(Cyto)iGlucoSnFR

Plasmid

#164513

Purpose

Green glucose sensor, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2 (Plasmid #244111) instead of this plasmid.

Depositor

Loren Looger

Article

Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

Insert

(Cyto)iGlucoSnFR

Use

AAV

Mutation

Synthetic Construct

Promoter

GFAP

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-mGreenLantern

Plasmid

#161913

Purpose

mGreenLantern fluorescent protein: bacterial expression plasmid

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

6xHis

Expression

Bacterial

Promoter

araB

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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p201B Cas9

Plasmid

#59177

Purpose

Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
BAR

Use

CRISPR

Promoter

2x35S

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Sst44-GFP

Plasmid

#172310

Purpose

SST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneurons

Depositor

Michael Greenberg

Article

Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

Insert

Sst44

Use

AAV

Promoter

pB-Globin

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-SENP5

Plasmid

#34555

Depositor

Mary Dasso

Article

Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

Insert

SENP5 (SENP5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only
pGL3-p27Luc

Plasmid

#23047

Depositor

William Kaelin

Article

Zhang et al Nat Med. 2004 Jun . 10(6):643-8.

Insert

p27 (CDKN1B Human)

Tags

Luciferase

Expression

Mammalian

Mutation

wt cDNA

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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MSCV let-7g

Plasmid

#14784

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

let-7g

Use

Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
P307-FBP_6

Plasmid

#139697

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pComb3XLambda

Plasmid

#63892

Purpose

Backbone pComb3X containing PCR template for human antibody lambda light chain constant region

Depositor

Carlos Barbas

Article

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

human antibody lambda light chain (IGLL5 Human)

Use

Phage display

Expression

Bacterial

Promoter

lacZ

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pET-Cre-6xHis

Plasmid

#62939

Purpose

Expression of Cre-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

Cre recombinase

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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Ef1a_NANOG_Ires_Puro

Plasmid

#18920

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

NANOG (NANOG Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pTriEx-mPA-GFP-PA-Rac1

Plasmid

#22022

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Avena sativa (oat), Human)

Tags

6X His and mPA-GFP

Expression

Bacterial, Insect, and Mamm…

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pActPL-VP16AD

Plasmid

#15305

Depositor

Benjamin White

Article

Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

Insert

VP16AD-ZIP+

Expression

Insect

Available Since

July 6, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-ArchT-WPREpA

Plasmid

#84472

Purpose

pAAV-RAM-ArchT

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

ArchT

Use

AAV

Tags

EGFP

Promoter

TRE

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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