12,469 results

1. pJET1.2_Rab28_Anti-Sense

   Plasmid

   #124442

   Purpose

   Plasmid for anti-sense in situ probe in vitro transcription

   Depositor

   Yingguang Frank Chan

   Article

   Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

   Insert

   RAB28, member RAS oncogene family (Rab28 Mouse)
   

   Use

   In situ probe

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJET1.2_Bod1l_Sense

   Plasmid

   #124443

   Purpose

   Plasmid for sense in situ probe in vitro transcription

   Depositor

   Yingguang Frank Chan

   Article

   Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

   Insert

   Biorientation of chromosomes in cell division 1-like (Bod1l Mouse)
   

   Use

   In situ probe

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCLXSN(GFP)-HA-Tbkbp1 1-330

   Plasmid

   #125179

   Purpose

   Retroviral expression of mouse Tbkbp1 (1-330)

   Depositor

   Shao-Cong Sun

   Article

   Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

   Insert

   Tbkbp1 (Tbkbp1 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-330

   Promoter

   CMV

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28B-PU.1-ETS-H3/S3

   Plasmid

   #124627

   Purpose

   Chimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffold

   Depositor

   Gregory Poon

   Article

   Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

   Insert

   PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Residues 237 to 241 replaced with corresponding s…

   Promoter

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28B-PU.1-ETS-S3

   Plasmid

   #124550

   Purpose

   Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

   Depositor

   Gregory Poon

   Article

   Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

   Insert

   PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Residues 242 to 246 replaced with corresponding s…

   Promoter

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28b-PU.1-ETS-H2

   Plasmid

   #123358

   Purpose

   Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

   Depositor

   Gregory Poon

   Article

   Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

   Insert

   PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Residues 207 to 215 replaced with corresponding s…

   Promoter

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5

   Plasmid

   #117137

   Purpose

   Expression vector encoding BFP-BSD-Nkx2-5

   Depositor

   Kosuke Yusa

   Article

   Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

   Insert

   Nkx2-5 CDS (Nkx2-5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   modified BFP

   Promoter

   human U6 promoter, human EF1a promoter

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP

   Plasmid

   #124181

   Purpose

   piggyBac-based reporter (Puro) driven by the Pou5f1 enhancer

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   IRES-Pac, Pou5f1 enhancer
   

   Use

   Reporter

   Tags

   Expression

   Mutation

   Promoter

   UBCmin

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZDonor-U6-myogenin stop codon gRNA

   Plasmid

   #69549

   Purpose

   Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.

   Depositor

   Charles Gersbach

   Insert

   Myogenin C terminus-targeting guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMX-eSOX2AHK

    Plasmid

    #118826

    Purpose

    Retroviral expression of a mutant of Sox2 that improves efficiency of iPSC generation

    Depositor

    Ralf Jauch

    Article

    Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

    Insert

    eSox2AHK (Sox2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    AHK: E46A, I53H

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMX-eSOX2NRR

    Plasmid

    #118825

    Purpose

    Retroviral expression of a mutant of Sox2 that improves efficiency of iPSC generation

    Depositor

    Ralf Jauch

    Article

    Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

    Insert

    eSOX2NRR (Sox2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    NRR: E46N, I53R, K57R

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMCh1750_S2F-IMCg_doxy-CMV_mChe-Cdc42E7

    Plasmid

    #118614

    Purpose

    expression of mChe-tagged cdc42E7 under doxy-inducible CMV promoter

    Depositor

    Marina Chekulaeva

    Article

    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

    Insert

    cdc42E7 (Cdc42 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    tetON CMV

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMCh1753_S2F-IMCg_doxy-CMV_mChe-Cdc42E7-E63'UTR

    Plasmid

    #118615

    Purpose

    expression of mChe-tagged cdc42E7-E6-3'UTR (swap) under doxy-inducible CMV promoter

    Depositor

    Marina Chekulaeva

    Article

    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

    Insert

    cdc42E7-E6 3'UTR (Cdc42 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    tetON CMV

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMCh1809_pBS_cdc42E6-boxB

    Plasmid

    #118609

    Purpose

    cdc42E6 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

    Depositor

    Marina Chekulaeva

    Article

    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

    Insert

    cdc42E6 3'UTR (Cdc42 Mouse)
    

    Use

    In vitro transcription

    Tags

    boxB

    Expression

    Mutation

    Promoter

    T3

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMCh1807_pBS_cdc42E7-boxB

    Plasmid

    #118612

    Purpose

    cdc42E7 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

    Depositor

    Marina Chekulaeva

    Article

    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

    Insert

    cdc42E7 3'UTR (Cdc42 Mouse)
    

    Use

    In vitro transcription

    Tags

    boxB

    Expression

    Mutation

    Promoter

    T3

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP

    Plasmid

    #124180

    Purpose

    piggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    EGFP-IRES-Pac, Pou5f1 enhancer
    

    Use

    Reporter

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psubCMV-WPRE-IL3SP-mVEGF-D-dNdC

    Plasmid

    #119229

    Purpose

    Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.

    Depositor

    Kari Alitalo

    Insert

    IL3SP-Vegfd-dNdC (Vegfd Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSiren_EGFP-shHmga2#2

    Plasmid

    #122290

    Purpose

    Knockdown for mouse Hmga2

    Depositor

    Yukiko Gotoh

    Article

    Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

    Insert

    Knockdown shRNA for mouse Hmga2 (Hmga2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHTSHP-ShhN-Cys

    Plasmid

    #121136

    Purpose

    Expresses ShhN with a C-terminal cysteine residues in bacteria

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    shh (Shh Mouse)
    

    Use

    Tags

    HIS, HRV3C site, and MBP

    Expression

    Bacterial

    Mutation

    Amino acid sequence from 26-191, with an addition…

    Promoter

    tac

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5 FLAG membrane-bound lipin-1 (delta200)

    Plasmid

    #120278

    Purpose

    Expression of ER/Golgi-tethered lipin-1 in mammalian cells

    Depositor

    Sirio Dupont

    Article

    Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

    Insert

    Lipin-1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG and in-frame fusion with the delta200 Golgi-…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Anti-REEP1/2 [N326D/29R]

    Plasmid

    #114510

    Purpose

    Mammalian Expression Plasmid of anti-REEP1/2 (Mouse). Derived from hybridoma N326D/29.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-REEP1/2 (Mus musculus) recombinant mouse monoclonal antibody (Reep1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Anti-KCNT2/Slo2.1/Slick K+ channel [N11/33R]

    Plasmid

    #114496

    Purpose

    Mammalian Expression Plasmid of anti-KCNT2/Slo2.1/Slick K+ channel (Mouse). Derived from hybridoma N11/33.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-KCNT2/Slo2.1/Slick K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnt2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Anti-PARIS/ZNF746 [N196/16R]

    Plasmid

    #114475

    Purpose

    Mammalian Expression Plasmid of anti-PARIS/ZNF746 (Human). Derived from hybridoma N196/16.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-PARIS/ZNF746 (Homo sapiens) recombinant mouse monoclonal antibody (ZNF746 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-NFIX2ERT2

    Plasmid

    #112704

    Purpose

    Expression of NFIX2-ERT2 fusion protein

    Depositor

    Richard Gronostajski

    Insert

    Nuclear Factor I X2 (Nfix Mouse)
    

    Use

    Tags

    ERT2 and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-U6-Diras2_2-hSyn::mCherry.3xFLAG-WPRE

    Plasmid

    #120394

    Purpose

    pAAV plasmid expressing Diras2 shRNA2 under the U6 promoter and mCherry.3XFLAG under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

    Insert

    Diras2 shRNA (Diras2 Mouse)
    

    Use

    AAV and RNAi

    Tags

    mCherry.3XFLAG

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CMV-Lin28a-as

    Plasmid

    #120528

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ELK1 A box-NLS pcDNA3.1/HisC

    Plasmid

    #121096

    Purpose

    Expression of the A box fragment of ELK1

    Depositor

    Paul Herring

    Article

    Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

    Insert

    ELK1 (Elk1 Mouse)
    

    Use

    Tags

    6XHis-Omni-xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ELK1 AB box pcDNA3.1/HisC

    Plasmid

    #121094

    Purpose

    Expression of the AB box fragment of ELK1

    Depositor

    Paul Herring

    Article

    Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

    Insert

    ELK1 (Elk1 Mouse)
    

    Use

    Tags

    6xHis-omni-express

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ELK1 B box-NLS pcDNA3.1/HisC

    Plasmid

    #121095

    Purpose

    Expression of the B box fragment of ELK1

    Depositor

    Paul Herring

    Article

    Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

    Insert

    ELK1 (Elk1 Mouse)
    

    Use

    Tags

    6XHis-Omni-xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. U6-Mos-as

    Plasmid

    #120534

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Mos (Mos Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    A>G (see depositor comments)

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge