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pDONR-IAA12_no stop

Plasmid

#83849

Purpose

Gateway donor vector for IAA12 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

NM_100334.4 (IAA12 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mCE

Plasmid

#82459

Purpose

Expression of myc epitope and murine mRNA Capping Enzyme in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mRNA capping enzyme (Rngtt Mouse)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pET24a_CDP-CHase

Plasmid

#73161

Purpose

Expresses in E. coli, Bacillus cereus NP_831800 CDP-CHase

Depositor

Maurice Bessman, Sandra Gabelli

Article

Duong-Ly et al J Bacteriol. 2011 Jul;193(13):3175-85. d

Insert

pET24a

Expression

Bacterial

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only
pJK359

Plasmid

#71570

Purpose

Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase (UctA)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

putative CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK286

Plasmid

#71567

Purpose

Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (UctB)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

formyl-CoA:oxalate CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK358

Plasmid

#71569

Purpose

Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase (UctD)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

putative CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-TRP32

Plasmid

#71730

Purpose

inducible expression of TRP32 in E.coli

Depositor

Jere McBride

Article

Luo et al Infect Immun. 2008 Apr;76(4):1572-80. do

Insert

TRP32

Tags

6xHIS and Thioredoxin

Expression

Bacterial

Promoter

pBAD

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_CUS1H02

Plasmid

#71070

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf11 (Taf11 Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
mRuby2-CytERM-N-17

Plasmid

#55895

Purpose

Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

CytERM

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pUC19 gene 60 thru gap + Cys

Plasmid

#49161

Purpose

Template for generating gene 60 mRNA truncated two codons after coding gap + Cys

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Mutation

gene 60 mRNA truncated two codons after coding ga…

Promoter

T7

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM1-C103A

Plasmid

#58419

Purpose

Expresses murine IFITM1-C103A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 103 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C83A

Plasmid

#58418

Purpose

Expresses murine IFITM1-C83A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 83 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C50A

Plasmid

#58417

Purpose

Expresses murine IFITM1-C50A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 50 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C49A

Plasmid

#58416

Purpose

Expresses murine IFITM1-C49A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 49 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pUAST myc Rab35 CA

Plasmid

#58382

Purpose

expression of drosophila myc-Rab35 constitutively active mutant

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Fly)

Tags

myc

Expression

Insect

Mutation

Q67L (constitutive active)

Promoter

GAL4

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pUAST YFP Rab35 CA

Plasmid

#58381

Purpose

expression of drosophila YFP-Rab35 constitutively active mutant

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Fly)

Tags

YFP

Expression

Insect

Mutation

Q67L (constitutive active)

Promoter

GAL4

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
Myc-CICf_AEA

Plasmid

#48188

Purpose

Expresses AEA mutated Cic

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Capicua (Cic Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-103 Y96F

Plasmid

#47094

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y96F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y96F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-103 Y32F

Plasmid

#47091

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y32F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y32F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 Y96F

Plasmid

#47085

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-09 Y96F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y96F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only