We narrowed to 15,548 results for: LEA

1. pCAG-rox-inv[Talpha1-iCre-pA]-rox-mNeonGreen-hCentrin1

   Plasmid

   #196892

   Purpose

   Neuron-specific expression of human (h)Centrin fused to mNeonGreen. Used in combination with Talpha1-Dre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mNeonGreen-hCentrin1 (CETN1 Human)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-hCentrin1

   Plasmid

   #196891

   Purpose

   Neuron-specific expression of human (h)Centrin fused to 2xHA-tags

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   2xHA-hCentrin1 (CETN1 Human)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBactin-Nedd1-mOrange2-rCM1

   Plasmid

   #196861

   Purpose

   Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mOrange2-rCM1 (Nedd1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBactin-Nedd1-mOrange2-F75A

   Plasmid

   #196862

   Purpose

   Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mOrange2-F75A (Nedd1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F75A in the CM1 domain

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBactin-Akna-mOrange2

   Plasmid

   #196865

   Purpose

   Expression of the centrosomal protein Akna fused to mOrange2

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akna-mOrange2 (Akna Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Myc-Nix-S35A

   Plasmid

   #197562

   Purpose

   Mammalian expression of myc-tagged human Nix with an alanine substitution of serine-35. Based on Myc-Nix (#100795)

   Depositor

   Joseph Gordon

   Article

   Field et al Autophagy. 2025 Jul;21(7):1544-1555. doi

   Insert

   BCL2 interacting protein 3 like (BNIP3L Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Serine 35 changed to alanine

   Promoter

   CMV

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Nmyc-Eps8L2_1-496-siR

   Plasmid

   #196515

   Purpose

   Expression of Myc-Eps8L2_1-496

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Eps8L2_1-496 (EPS8L2 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Silent mutations for siRNA resistance

   Promoter

   T7 promoter

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Myc-Nix-S35D

   Plasmid

   #197563

   Purpose

   Mammalian expression of myc-tagged human Nix with an aspartic acid substitution of serine-35. Based on Myc-Nix (#100795)

   Depositor

   Joseph Gordon

   Article

   Field et al Autophagy. 2025 Jul;21(7):1544-1555. doi

   Insert

   BCL2 interacting protein 3 like (BNIP3L Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Serine 35 changed to aspartic acid

   Promoter

   CMV

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-dMET

   Plasmid

   #176031

   Purpose

   A knock-out vector for dog MET

   Depositor

   Michiyuki Matsuda

   Article

   Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

   Insert

   A gRNA targeting the dog MET gene and the cDNA of Cas9 (MET )
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-NLS-YFP-CCTc

    Plasmid

    #184327

    Purpose

    Mammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.2)

    Depositor

    Xiaodong Liu

    Article

    Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

    Insert

    CCTc (CACNA1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHsSyx3-MBP

    Plasmid

    #179286

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Use

    Tags

    TEV protease cleavage site + mature maltose bindi…

    Expression

    Bacterial

    Mutation

    residues 393-792 from accession number NP_0010361…

    Promoter

    T7

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMBP-HsSyx3

    Plasmid

    #179287

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Use

    Tags

    His6 + mature maltose binding protein + TEV prote…

    Expression

    Bacterial

    Mutation

    residues 393-792 from accession number NP_0010361…

    Promoter

    T7

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMBP-HsSyx-375-792

    Plasmid

    #179291

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Use

    Tags

    His6 + mature maltose binding protein + TEV prote…

    Expression

    Bacterial

    Mutation

    residues 375-792 from accession number NP_0010361…

    Promoter

    T7

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MSCV-HA-Nr4a1-Zf2

    Plasmid

    #160944

    Purpose

    Generation of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 2 deletion)

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Nr4a1 (Nr4a1 Mouse)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Deletion of Zinc finger 2 (C306 to C330)

    Promoter

    MSCV-LTR

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-HA-Nr4a1-Zf1

    Plasmid

    #160943

    Purpose

    Generation of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 1 deletion)

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Nr4a1 (Nr4a1 Mouse)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Deletion of Zinc finger 1 (C270 to C290)

    Promoter

    MSCV-LTR

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-hPC1_MTS_L4139P-eGFP-3HA

    Plasmid

    #108404

    Purpose

    Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    3xHA and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-mPC1_4119-eGFP

    Plasmid

    #108394

    Purpose

    Genomic region, including intron 45, to express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    variant ID rs13468401 missense variant R4237Q

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mature ISG15 C-term. Domain (79-165, W123A)

    Plasmid

    #110765

    Purpose

    Bacterial expression of mutant proISG15 C-term. domain

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    proISG15 C-term. domain (79-165, W123A) (ISG15 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    W123A

    Promoter

    T7

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4/TO h-Stx3 L165A/E166A-2xMyc/His

    Plasmid

    #99743

    Purpose

    Expresses human Stx3 locked into the open conformation with a C-term myc-myc-his tag in mammalian cells.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

    Insert

    Stx3 (STX3 Human)
    

    Use

    Tags

    myc-myc-his

    Expression

    Mammalian

    Mutation

    Changed both L165 and E166 to Alanine

    Promoter

    CMV

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-mPC1_4148-3HA

    Plasmid

    #108398

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    3HA

    Expression

    Mammalian

    Mutation

    variant ID rs13468401 missense variant R4237Q

    Promoter

    CMV

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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