We narrowed to 88,163 results for: MAL

1. MSCV eIF4E IRES GFP

   Plasmid

   #18761

   Depositor

   Scott Lowe

   Article

   Wendel et al Nature. 2004 Mar 18. 428(6980):332-7.

   Insert

   eIF4E (Eif4e Mouse)
   

   Use

   Retroviral

   Tags

   IRES GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pL-SFFV.Reporter.RFP657.PAC

   Plasmid

   #61395

   Purpose

   Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPE3-pPuro

   Plasmid

   #215722

   Purpose

   Cloning vector for Uni-Vector prime editing system

   Depositor

   Kou-Juey Wu

   Article

   Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

   Insert

   PE2
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFX-mCherry-Rab11

   Plasmid

   #174461

   Purpose

   Express mCherry with recycling endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with recycling endosome-targeting sequence
   

   Use

   Tags

   small GTPase (Rab11)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

   Plasmid

   #45579

   Purpose

   Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   coY2 Ani K227M
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTEV5_ RpMatB_2

   Plasmid

   #78206

   Purpose

   Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding

   Depositor

   Martin Webb

   Article

   Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

   Insert

   matB (His6 C106A S170A R286C Q457C K488A)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C106A S170A R286C Q457C K488A

   Promoter

   T7

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST

   Plasmid

   #175019

   Purpose

   Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

   Insert

   D/bovine/Nebraska/9-5/2012
   

   Use

   Tags

   GCN4-TEV-sfGFP-TwinStrep

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PGK+ plasmid with onboard mCherry cassette

   Plasmid

   #169745

   Purpose

   PGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK-tetO2

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPRIME-CMV-dsRed-FF3

   Plasmid

   #11664

   Purpose

   3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of dsRed and miR30-based shRNA targeting firefly luciferase

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Insert

   FF3
   

   Use

   Lentiviral

   Tags

   dsRed

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA4-Beclin1 1-242

    Plasmid

    #24391

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-242.

    Promoter

    Available Since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pFX-mCherry-Rab7

    Plasmid

    #174458

    Purpose

    Express mCherry with late endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with late endosome-targeting sequence
    

    Use

    Tags

    small GTPase (Rab7)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-CRY2-mCh-superPLDx10

    Plasmid

    #188986

    Purpose

    A lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type)

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Use

    Tags

    CRY2 and mCherry

    Expression

    Mammalian

    Mutation

    F163L, K249R, G328S, G381V, G429D, D480G

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-CMV-TetO2_mTurquoise2

    Plasmid

    #113895

    Purpose

    Mammalian (inducible) expression of cytosolic mTurquoise2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    monomeric Turquoise2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMito-miRFP718nano

    Plasmid

    #195754

    Purpose

    Mitochondria labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    Human cytochrome C oxidase subunit VIII-miRFP718nano
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-StrepHA-SUMO2(Q87R)

    Plasmid

    #66868

    Purpose

    Mammalian expression of SUMO2(Q87R) with a strep and an HA tag

    Depositor

    Ying Liu

    Article

    Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

    Insert

    SUMO2 (SUMO2 Human)
    

    Use

    Tags

    HA and Strep

    Expression

    Mammalian

    Mutation

    Q87R

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CaMKIIa-Archer1-EGFP

    Plasmid

    #60423

    Purpose

    Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing Archer1-EGFP fusion. Archer1's red fluorescence monitors changes in membrane voltage.

    Depositor

    Viviana Gradinaru

    Article

    Flytzanis et al Nat Commun. 2014 Sep 15;5:4894. doi: 10.

    Insert

    Archer1-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6T7

    Plasmid

    #71462

    Purpose

    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)

    Depositor

    Willie Mark

    Article

    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    human U6/phage T7

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBK-EQR-BE3

    Plasmid

    #85172

    Purpose

    Expresses EQR-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    EQR-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pH2B-miRFP718nano

    Plasmid

    #195750

    Purpose

    Histone 2B labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    H2B-miRFP718nano
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

    Plasmid

    #167898

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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