We narrowed to 7,952 results for: Lif
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Plasmid#49859PurposeEncodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tagDepositorInsertGJA1-20k (GJA1 Human)
TagsV5ExpressionMammalianMutationGJA1-20k fragment corresponds to residues 213-382…PromoterCMVAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S2-RAB_EKARev
Plasmid#160726PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.DepositorInsertS2-RAB_EKARev
UseAAVExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-hDND1
Plasmid#70070PurposeGateway entry vector for human DND1 with stop codonDepositorInsertDnd1 (DND1 Human)
UseGateway CloningAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-MDMX (1-120)
Plasmid#62065PurposeExpression of human MDM2 (1-120) in e. coliDepositorInsertMDM4 (MDM4 Human)
TagsGSTExpressionBacterialMutationcontains residues 1-120PromotertacAvailable SinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pR26-mBMP7
Plasmid#127374PurposeAllows for doxycycline-inducible BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorInsertBMP7 (Bmp7 Mouse)
UseCRISPR and Mouse TargetingAvailable SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S3-RAB_EKARev
Plasmid#160727PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.DepositorInsertS3-RAB_EKARev
UseAAVExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-FUSN-miRFP670-Cry2WT
Plasmid#122441PurposeExpresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WTDepositorInsertFUS (FUS Human)
UseLentiviralTagsCry2WT and miRFP670ExpressionMammalianMutationContains only amino acids 1-214PromoterSFFVAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains1
Plasmid#78461PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
TagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2149-2170; Q2513R (please see…PromoterT7Available SinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains2
Plasmid#78460PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
TagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2264-2315; Q2513R (see deposi…Available SinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S1-jRCaMP1a
Plasmid#160728PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.DepositorInsertS1-jRCaMP1a
UseAAVExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-NoStart
Plasmid#49853PurposeEncodes human connexin 43 with the start methionine codon mutated to isoleucine and with wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
ExpressionMammalianMutationStart codon mutated to from methionine to isoleuc…PromoterCMVAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-NSF/S647A
Plasmid#74938PurposeMammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)DepositorInsertNSF (NSF Human)
TagsFLAGExpressionMammalianMutationS647A (mutation in the D2 domain of the protein)PromoterCMVAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-LiCre
Plasmid#219797PurposeExpresses a codon-optimized His-tagged Light-inducible Cre (LiCre) recombinase in E. coli. Plasmid ID in Yvert lab is pGY611. LiCre is described in https://doi.org/10.7554/eLife.61268DepositorInsertCodon-optimized Light-inducible Cre Recombinase
UseCre/LoxTagsHIS-tag for purificationExpressionBacterialPromoterIPTG-inducibleAvailable SinceMay 20, 2026AvailabilityAcademic Institutions and Nonprofits only -
pART27::CLOMELEON
Plasmid#247988PurposeStable expression of the indicator protein CLOMELEON for monitoring chloride concentrations in the cytoplasm of plant cells.DepositorInsertCLOMELEON (CFP-YFP fusion with ENLYFQG-linker)
Tags6xHisExpressionPlantMutationCFP-variant: K26R, F64L, S65T, Y66W, N146I, M153T…PromoterCauliflower Mosaic Virus 35S promoter (GenBank AJ…Available SinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
Mirror GFP[ENE(C8351G)-mascRNA](pAVA3000)
Plasmid#239349PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of C8351G MALAT1 ENE-mascRNA reporter.DepositorInsertMALAT1 ENE(C8351G)-mascRNA
ExpressionMammalianMutationMALAT1 ENE(C8351G)Available SinceAug. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
Mirror RFP[ENE(C8351G)-mascRNA](pAVA2995)
Plasmid#239350PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of C8351G MALAT1 ENE-mascRNA reporter.DepositorInsertMALAT1 ENE(C8351G)-mascRNA
ExpressionMammalianMutationMALAT1 ENE(C8351G)Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAH33
Plasmid#226850PurposeTo constitutively overexpress Opa1 from the safe harbor locus Rosa26 in MEFDepositorAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-FRB-KRas/N12
Plasmid#214302PurposeExpress split-KRas fragment fused with FRB and MBPDepositorInsertSplit-KRas fragment fused with FRB (KRAS Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialPromoterTacAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-KRas/13C-FKBP-MBP
Plasmid#214303PurposeExpress split-KRas fragment fused with FKBP and MBPDepositorInsertSplit-KRas fragment fused with FKBP (KRAS Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationKRas-Q61LPromoterT7Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR
Plasmid#207135PurposeDNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-inDepositorInsert5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
ExpressionMammalianAvailable SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only