We narrowed to 8,173 results for: 11

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pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP

Plasmid

#227011

Purpose

Lentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP

Plasmid

#227012

Purpose

Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Promoter

UbC

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-nucGFP11-3xPax7gRNA

Plasmid

#224570

Purpose

Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

GFP11-H2B

Use

CRISPR

Tags

Histone H2B (nuclear localization)

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-3xGFP11-mem-3xPax7gRNA

Plasmid

#224571

Purpose

Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

3xGFP11

Use

CRISPR

Tags

Membrane Localization Signal

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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L2111-FKBP-GPA-20-11S

Plasmid

#222882

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc11S

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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L2379-FKBP-GPA(V84R)-20-11S

Plasmid

#222901

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA(V84R)-20GS-NanoLuc11S

Tags

Myc

Expression

Mammalian

Mutation

V84R in GPA

Promoter

CMV

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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L2375-FKBP-GPA-20-11S

Plasmid

#222897

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc11S

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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L2378-FRB-GPA(V84R)-20-11S

Plasmid

#222899

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA(V84R)-20GS-NanoLuc11S

Tags

Myc

Expression

Mammalian

Mutation

V84R in GPA

Promoter

CMV

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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L2374-FRB-GPA-20-11S

Plasmid

#222895

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc11S

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135

Plasmid

#222875

Purpose

Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA(WT)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138

Plasmid

#222876

Purpose

Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

FRB chain: V84R in GPA

Promoter

MSCV LTR

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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L2109-FRB-GPA-20-11S

Plasmid

#222880

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc11S

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b I-SceI (Ctermhis) K223I

Plasmid

#195615

Purpose

Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity

Depositor

Frederick Gimble

Article

Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

Insert

I-SceI

Expression

Bacterial

Mutation

C2694G, G2345C, G2351C, A3365T, A3366T

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b I-SceI (Ctermhis) K122I

Plasmid

#195614

Purpose

Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity

Depositor

Frederick Gimble

Article

Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

Insert

I-SceI

Expression

Bacterial

Mutation

C2458G, A2826T, A2827T

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-2X(CMV-eBFP2-3XNLS-pA)

Plasmid

#195867

Purpose

to express nuclear localized eBFP2

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

eBFP2

Use

AAV

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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His-MBP-IST1 N-terminal domain

Plasmid

#184805

Purpose

Bacterial expression of IST1 N-terminal domain

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

IST1 (IST1 Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

aa 1-187 only

Promoter

T7

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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KBB428

Plasmid

#185090

Purpose

NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

First nuclear localization signal mutated (amino …

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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His-MBP-CHMP1B, S2C

Plasmid

#184803

Purpose

Bacterial expression of CHMP1B (S2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

CHMP1B (CHMP1B Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

S2C

Promoter

T7

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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MTF2_KO_ex11_gRNA-4

Plasmid

#131334

Purpose

gRNA to knockout MTF2. This gRNA is also used in Haojie Li et al. Nature 2017. It targets exon 11 of MTF2.

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

MTF2_KO_ex11_gRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry NEDD4 WW2 neg ctrl

Plasmid

#112030

Purpose

Mode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Insert

NEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry

Tags

Split mCherry

Expression

Bacterial

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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