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EC.SurA.pTYB1.a21-428.M231R

Plasmid

#15956

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

mature SurA protein M231R mutant

Tags

intein

Expression

Bacterial

Mutation

M231R

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
PA1024_pET21a(+)C-tag

Plasmid

#14579

Depositor

SeWon Suh

Article

Ha et al J Biol Chem. 2006 Jul 7. 281(27):18660-7

Insert

2-nitropropane dioxygenase (PA1024 Pseudomonas aeruginosa)

Tags

His

Expression

Bacterial

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only
pBS 14-3-3 zeta

Plasmid

#19226

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Tyr3/Trp5 monooxygenase (YWHAZ Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pJC45BS104 L553W

Plasmid

#1306

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L553W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 553 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pJB127

Plasmid

#248630

Purpose

Theophylline-based control of repA on a C. difficile plasmid for use in allelic exchange and codA added to help cure strains of the plasmid.

Depositor

Joseph Sorg

Article

Brehm et al Anaerobe. 2024 Apr 29;88:102858. doi: 10

Inserts

sacB (sacB )
codA

Use

Other

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pE-SUMO_PspCas13b

Plasmid

#252295

Purpose

Expresses PspCas13b (WT) in bacterial cells.

Depositor

Hiroshi Nishimasu

Article

Ishikawa et al Nat Struct Mol Biol. 2025 Aug;32(8):1567

Insert

PspCas13b (WT)

Expression

Bacterial

Mutation

WT

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pCL0981

Plasmid

#252427

Purpose

Mitochondria-tethered construct for the RAPID-Release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

Histone H4 (H4C6 Human)

Tags

EGFP-TVMVx2-FRB-OMP25

Expression

Mammalian

Mutation

Truncation: aa 47-102 only

Promoter

CMV

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCL0918

Plasmid

#252411

Purpose

Mitochondria-tethered construct for the RAPID-Release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

Histone H4 (H4C6 Human)

Tags

EGFP-TVMVx2-FRB-OMP25

Expression

Mammalian

Mutation

Truncation: aa 1-46 only

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCL0946

Plasmid

#252416

Purpose

Mitochondria-tethered construct for the RAPID-Release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

Histone H4 (H4C6 Human)

Tags

EGFP-TVMVx2-FRB-OMP25

Expression

Mammalian

Mutation

Truncation: aa 1-20 only

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pEW003

Plasmid

#245129

Purpose

Based on pAF259 but with bitluc expression driven by the C. saccharoperbutylacetonicum flgB promoter.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

PflgB-bitlucopt

Expression

Bacterial

Promoter

PflgB

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pEW004

Plasmid

#245130

Purpose

Based on pAF259 but with bitluc expression driven by the C. saccharoperbutylacetonicum Cspa_RS22190 promoter.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Pcspa_RS22190 -bitlucopt

Expression

Bacterial

Promoter

Pcspa_RS22190

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pEW005

Plasmid

#245131

Purpose

Based on pAF259 but with bitluc expression driven by the C. saccharoperbutylacetonicum rpoD promoter.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

PrpoD-bitlucopt

Expression

Bacterial

Promoter

PrpoD

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pEW007

Plasmid

#245132

Purpose

Based on pAF259 but with bitluc expression driven by the C. saccharoperbutylacetonicum Cspa_135p00700 promoter.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Pcspa_135p00700-bitlucopt

Expression

Bacterial

Promoter

Pcspa_135p00700

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pLLK010

Plasmid

#245133

Purpose

Based on pAF259 but with bitluc expression driven by the C. difficile minimal slpA promoter.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

PslpAmin-bitlucopt

Expression

Bacterial

Promoter

PslpAmin

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pLLK011

Plasmid

#245134

Purpose

Based on pAF259 but with bitluc expression driven by the C. difficile minimal slpA promoter with an additional 37bp upstream.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

PslpAmid-bitlucopt

Expression

Bacterial

Promoter

PslpAmid

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pLLK012

Plasmid

#245135

Purpose

Based on pAF259 but with bitluc expression driven by the C. difficile minimal slpA promoter with an additional 332bp upstream.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

PslpAlong-bitlucopt

Expression

Bacterial

Promoter

PslpAlong

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK081

Plasmid

#245144

Purpose

Knock-in plasmid for insertion of DNA sequences between CD0188 (pyrE) and CD0189 in the C. difficile R20291 genome.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Ptet-mreB2

Use

Bacterial knock-in

Promoter

Ptet

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK122

Plasmid

#245145

Purpose

Expression plasmid with the constitutive Pcwp2 promoter driving expression of mCherry.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Pcwp2-mCherry

Expression

Bacterial

Promoter

Pcwp2

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pLLK007

Plasmid

#245126

Purpose

Control plasmid based on pAF259 (Addgene #105494). Contains a promoterless luciferase-encoding bitluc.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Bitlucopt

Expression

Bacterial

Promoter

None

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pEW001

Plasmid

#245127

Purpose

Based on pAF259 but with bitluc expression driven by the C. saccharoperbutylacetonicum bdh promoter.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Pbdh-Bitlucopt

Expression

Bacterial

Promoter

Pbdh

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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