We narrowed to 2,359 results for: T2A

1. pSH-Csy4-T2A-SaRFN

   Plasmid

   #85755

   Purpose

   Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Insert

   Csy4-T2A-FokI-SadCas9
   

   Use

   CRISPR

   Tags

   3xHA tag, Csy4, and FokI fusion via 36 amino acid…

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   CAG

   Available since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDRF1-GW Electra1-T2A-mCherry

   Plasmid

   #205498

   Purpose

   Expression of Electra1-T2A-mCherry in yeast

   Depositor

   Bas Teusink

   Insert

   Electra1-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDRF1-GW eGFP-T2A-mCherry

   Plasmid

   #118422

   Purpose

   Produces equimolar expression of eGFP and mCherry

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   eGFP-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-pac-T2A-iCre

   Plasmid

   #113849

   Purpose

   Puromycin resistance gene and iCre recombinase

   Depositor

   Mario Capecchi

   Insert

   pac-T2A-iCre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDRF1-GW mKate2-T2A-mTurquoise2

   Plasmid

   #118426

   Purpose

   Produces equimolar expression of mKate2 and mTq2

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   mKate2-T2A-mTq2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mScarlet-I-T2A-LSS-SGFP2

   Plasmid

   #112946

   Purpose

   Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

   Depositor

   Dorus Gadella

   Insert

   mScarlet-I and LSS-SGFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF1α-M2rtTA-T2A-Puro

   Plasmid

   #169175

   Purpose

   piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174

   Depositor

   Igor Slukvin

   Article

   Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

   Insert

   M2rtTA-T2A-Puro
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Promoter

   EF1a

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. H2B-sfGFP-T2A-iCre

   Plasmid

   #178546

   Purpose

   pCMV-Histone H2B-sfGFP-T2A-iCre-bpA-FRT-Neo*-FRT

   Depositor

   Mario Capecchi

   Insert

   Histone H2B-sfGFP-T2A-iCre
   

   Use

   Cre/Lox and Mouse Targeting; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCVL iRFP-T2A-Trex2

   Plasmid

   #50418

   Purpose

   Coexpresses Trex2 and iRFP in mammalian cells.

   Depositor

   Andrew Scharenberg

   Inserts

   iRFP
   Trex2
   

   Use

   Lentiviral

   Tags

   T2A

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-mTagBFP2-T2A-iCreERT2 (PA)

    Plasmid

    #128174

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PA N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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