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pHAGE2 GFP-Rtn4HD

Plasmid

#86686

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

Rtn4 homology domain (RTN4 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

homology domain only

Promoter

CMV

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-hARL1(Q71L)

Plasmid

#206407

Purpose

Mammalian expression of hARL1(Q71L)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

ARL1 (ARL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q71L

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-hARL1(T31N)

Plasmid

#206408

Purpose

Mammalian expression of hARL1(T31N)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

ARL1 (ARL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T31N

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pmApple-EGFP-HRas

Plasmid

#133854

Purpose

Mammalian expression of HRas N-terminally tagged with mApple and EGFP

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

HRas (HRAS Human)

Use

Tags

EGFP and mApple

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 P294A

Plasmid

#112102

Purpose

Mammalian expression of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

P294A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-hs-ALPS 1-ALPS2-EGFP

Plasmid

#187113

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000101199 (ARFGAP1 Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET21b GFP-Rab10 Q68LHis

Plasmid

#186015

Purpose

GFP-Rab10 Q68LHis

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

GFP-Rab10 Q68LHis (RAB10 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-EGFP-ERas delCAAX-IP

Plasmid

#13834

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas delCAAX (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Deletion 4 amino acids of c-terminal end (CAAX mo…

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-EGFP-ERas SSVA-IP

Plasmid

#13827

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas SSVA (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C224 changed to serine

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pMRX-IP-EGFP-LOV2-OMP25

Plasmid

#233346

Purpose

Stable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

LOV2 domain of phototropin1
Omp25 (Synj2bp Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N-terminus is deleted and N538E

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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