Addgene

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DCT.VV.Orf71

Plasmid

#12759

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf71

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf95

Plasmid

#12783

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf95

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf187

Plasmid

#12875

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf187

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf74

Plasmid

#12762

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf74

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
Flag-ZDHHC22

Plasmid

#231670

Purpose

Expresses human ZDHHC22 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC22 (ZDHHC22 Human)

Use

Tags

3×Flag Tag

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC14

Plasmid

#231662

Purpose

Expresses human ZDHHC14 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC14 (ZDHHC14 Human)

Use

Tags

3×Flag Tag

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC13

Plasmid

#231661

Purpose

Expresses human ZDHHC13 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC13 (ZDHHC13 Human)

Use

Tags

3×Flag Tag

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-ZDHHC5

Plasmid

#231654

Purpose

Expresses human ZDHHC5 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC5 (ZDHHC5 Human)

Use

Tags

3×Flag Tag

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5-ΔJ (Δ14-82)

Plasmid

#205719

Purpose

Mammalian expression of DNAJC5-ΔJ (Δ14-82)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

Expression

Mammalian

Mutation

Δ14-82

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5-L115R

Plasmid

#205708

Purpose

Mammalian expression of DNAJC5 L115R

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

Expression

Mammalian

Mutation

L115R

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5-L116Δ

Plasmid

#205709

Purpose

Mammalian expression of DNAJC5 L116Δ

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

Expression

Mammalian

Mutation

L116Δ

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pJR017

Plasmid

#198835

Purpose

In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::TeTX-SL2-mKate::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJR032

Plasmid

#198773

Purpose

Stabilized step function Opsins ChR2 variant

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::hChR2 (C128S, D156A)-EYFP::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJR035

Plasmid

#198821

Purpose

For tissue specific RNA-seq

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::rpl-22HA-SL2-mKate::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHW491

Plasmid

#198808

Purpose

In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::Tetx cDNA::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL049

Plasmid

#198809

Purpose

In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::GCaMP6s-SL2-mKate2::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJR031

Plasmid

#198825

Purpose

Stable step function ChR2 variant. Activated by bluelight, terminated by green light.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::ChR2(C128S)-EYFP::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJR034

Plasmid

#198829

Purpose

Step-Waveform Inhibitory Channelrhodopsin

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::SwiCHR-TS-EYFP::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJR033

Plasmid

#198822

Purpose

Chloride-conducting channelrhodopsin

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::UAS-iC1-c2-TS-EYFP::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMP71-mGFP-llo190-mc38minor

Plasmid

#174604

Purpose

expresses palmitoylated GFP with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes Aatf, irgq and cpne1

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

palmitoyl-GFP C-term fusion LLO190 and minigenes of 3 neoantigens from MC38 tumor in genes Aatf irgq cpne1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1816

Plasmid

#154320

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1827

Plasmid

#154323

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1841

Plasmid

#154338

Purpose

Promoter reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR

Use

Tags

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-HA-mIFITM3-H136T

Plasmid

#58413

Purpose

Expresses murine IFITM3-H136T with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Histidine 236 to Threonine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf199

Plasmid

#12887

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf199

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf200

Plasmid

#12888

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf200

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf201

Plasmid

#12889

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf201

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf202

Plasmid

#12890

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf202

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf205

Plasmid

#12893

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf205

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf206

Plasmid

#12894

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf206

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only