We narrowed to 24,588 results for: Nes

1. pWN062

   Plasmid

   #233822

   Purpose

   MoClo-YTK; type3A; NS3 systems; LexA-NES

   Depositor

   Jeff Hasty

   Article

   O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

   Insert

   LexA-NES
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEM03

   Plasmid

   #135417

   Purpose

   Expression of Hygromycin resistance from Spizellomyces Hsp70 promoter

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   SpunHsp70pr
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Hsp70pr

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNB693

   Plasmid

   #60145

   Purpose

   Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   YeLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available Since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNB689

   Plasmid

   #60133

   Purpose

   Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   GrLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNB756

   Plasmid

   #60149

   Purpose

   Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   YeLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   LEU1pr

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNB752

   Plasmid

   #60155

   Purpose

   Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by ADE17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   GrLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   ADE17pr

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNB812

   Plasmid

   #60160

   Purpose

   Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   yEVenus-PEST
   

   Use

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   Promoter

   RNR1pr

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR v2

   Plasmid

   #52961

   Purpose

   Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hsyn-NE2m

   Plasmid

   #208686

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons

   Depositor

   Yulong Li

   Insert

   GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hsyn-NE2h

    Plasmid

    #208687

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neurons

    Depositor

    Yulong Li

    Has Service

    AAV5

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCas9n(D10A)-Blast

    Plasmid

    #63593

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, nickase mutant

    Promoter

    EFS-NS

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

    Plasmid

    #138152

    Purpose

    Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    eGFP-MCS
    

    Use

    Lentiviral

    Tags

    2PEST AND NLS

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDN77

    Plasmid

    #61347

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS2 and NES IkBa

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDN41

    Plasmid

    #61344

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS9 and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-FLAG-Claspin

    Plasmid

    #12659

    Depositor

    Michele Pagano

    Article

    Peschiaroli et al Mol Cell. 2006 Aug 4. 23(3):319-29.

    Insert

    Claspin (CLSPN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 16, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pGI3EM11

    Plasmid

    #135483

    Purpose

    Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunH2Bpr hph-GFP ADH1t
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2Bpr

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Integrin-αv Headpiece M400GC

    Plasmid

    #159637

    Purpose

    Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400

    Depositor

    Timothy Springer

    Article

    Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

    Insert

    alpha v headpiece M400GC
    

    Use

    Tags

    6x His and Strep II

    Expression

    Mammalian

    Mutation

    M400C

    Promoter

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGI3EM18

    Plasmid

    #135484

    Purpose

    Agrobacterium T-DNA with SpunH2Bpr driving hph-tdTomato

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunH2Bpr hph-tdTomato ADH1t
    

    Use

    Tags

    tdTomato

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2Bpr

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCK364.306

    Plasmid

    #192642

    Purpose

    Expresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    J306 scRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    BBa_J23119

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pminiTol2-HuC-NE2h

    Plasmid

    #208689

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h pan-neuronally in zebrafish

    Depositor

    Yulong Li

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Huc

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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