We narrowed to 12,077 results for: ache

1. pGGW_SM_BglII

   Plasmid

   #49314

   Purpose

   Subcloning vector for Golden Gate-based site-directed mutagenesis; BglII and KpnI to release the insert

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGGEV_2'_Linker

   Plasmid

   #49288

   Purpose

   Golden Gate entry vector at position 2, small linker insert, final entry vector for the assembly

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGGEV_4'_Linker

   Plasmid

   #49290

   Purpose

   Golden Gate entry vector at position 4, small linker insert, final entry vector for the assembly

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGGEV_6'_Linker

   Plasmid

   #49292

   Purpose

   Golden Gate entry vector at position 6, small linker insert, final entry vector for the assembly

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGGEV_7'_Linker

   Plasmid

   #49293

   Purpose

   Golden Gate entry vector at position 7, small linker insert, final entry vector for the assembly

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGGEV_8'_Linker

   Plasmid

   #49294

   Purpose

   Golden Gate entry vector at position 8, small linker insert, final entry vector for the assembly

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGGEV_+1_XcmI-LacZ

   Plasmid

   #49295

   Purpose

   Golden Gate entry vector at position 1, plus 5' ATG, LacZ

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Spectrin-M-cpstFRET

   Plasmid

   #61109

   Purpose

   Expression of Spectrin-cpstFRET to measure mechanical stress and report multidimensional stress fields

   Depositor

   Fred Sachs

   Article

   Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

   Insert

   spectrin-cpstFRET
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Spectrin-C-cpstFRET

   Plasmid

   #61111

   Purpose

   Expression of Spectrin-C-term cpstFRET to measure mechanical stress and report multidimensional stress fields

   Depositor

   Fred Sachs

   Article

   Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

   Insert

   spectrin-cpstFRET
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mTagBFP2-Lysosomes-20

    Plasmid

    #55308

    Purpose

    Localization: Lysosome Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    LAMP1
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pGL3-Basic-8x-gRNA-eGFP

    Plasmid

    #60718

    Purpose

    eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPSmOrange-N1

    Plasmid

    #31898

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

    Insert

    PSmOrange
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETM43 p62 FL (1-440)

    Plasmid

    #210425

    Purpose

    Bacterial expression of MBP-p62 FL (1-440) with 3C cleavage site

    Depositor

    Carsten Sachse

    Article

    Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

    Insert

    p62 FL (NUP62 Human)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBAD-PSmOrange

    Plasmid

    #31886

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

    Insert

    PSmOrange
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR-p14FAST

    Plasmid

    #50025

    Purpose

    pENTR vector for reptilian orthoreovirus p14 fusion-associated small transmembrane (FAST) protein

    Depositor

    Jamie Davies

    Article

    Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

    Insert

    p14FAST
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LV-TRE-Empty-T2A-dsRedExpress2

    Plasmid

    #60623

    Purpose

    Expresses dsRedExpress2 in response to doxycycline

    Depositor

    Charles Gersbach

    Article

    Kabadi et al ACS Synth Biol. 2014 Dec 10.

    Insert

    Empty
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPSmOrange-C1

    Plasmid

    #31899

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

    Insert

    PSmOrange
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid

    #69546

    Purpose

    Tet-On system expressing MyoD-2A-GFP-2xNLS.

    Depositor

    Charles Gersbach

    Inserts

    eGFP
    Myod1 (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    2x nuclear localization sequence and T2A "sk…

    Expression

    Mutation

    Removed stop codon to fuse it to the 2A-eGFPnls i…

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pH2B-PSmOrange

    Plasmid

    #31920

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

    Insert

    PSmOrange
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

    Promoter

    Available since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTagBFP2-MyosinIIA-C-18

    Plasmid

    #55315

    Purpose

    Localization: Cytoskeleton, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    MyosinIIA
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits