We narrowed to 4,234 results for: gca

1. pMS59

   Plasmid

   #215680

   Purpose

   Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA

   Plasmid

   #194247

   Purpose

   Expresses 3 miRNAs targeting human alpha-Synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   miRaSynuclein(human) (SNCA Human)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CROP_C9_Puro_VEGFB

   Plasmid

   #183328

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets VEGFB gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   VEGFB
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA

   Plasmid

   #194248

   Purpose

   Expresses 3 miRNAs targeting mouse alpha-Synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   miRaSynuclein(mouse) (Snca Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAN-PBAD-sgRNA-scramble

   Plasmid

   #62285

   Purpose

   expression of scramble sgRNA from the arabinose-inducible promoter

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   scramble sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTX1-EV71-SL2

   Plasmid

   #126043

   Purpose

   In-vitro-transcription of Enterovirus 71 5UTR-SL2 RNA in NMR tube for "Systems NMR" analysis

   Depositor

   Frédéric Allain

   Article

   Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

   Insert

   Enterovirus 71 5'UTR SL2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSP571

   Plasmid

   #139498

   Purpose

   CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA

   Depositor

   Serge Pelet

   Article

   Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

   Insert

   pGPD Cas9 / sgRNA (STL162)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   pGPD

   Available since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-U6-AasgRNA3.8.3

   Plasmid

   #121959

   Purpose

   Mammalian expression, Transcription regulation, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AaCas12b single chimeric gRNA, MS2 hairpin inserted
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAN-PBAD-sgRNA-A3T

   Plasmid

   #62260

   Purpose

   expression of A3T sgRNA from the arabinose-inducible promoter

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   A3T sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYAMTrGCsgScLEU2

    Plasmid

    #224869

    Purpose

    Disruption of S. cerevisiae type LEU2 genes

    Depositor

    Katsunori Suzuki

    Article

    Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

    Insert

    pYAMTrGCas having the guide sequence ScLEU2 (LEU2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMS77

    Plasmid

    #215681

    Purpose

    Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    eef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC57-ITR2-H1-GFP sgRNA Target 3- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210739

    Purpose

    Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 3

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    Sp Cas9 (cas9 S pyogenes)
    Sp sgRNA GFP Target 3
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    Promoter

    H1 and eCMV

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57-ITR2-H1-GFP sgRNA Gollum Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210747

    Purpose

    Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 3

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Gollum sgRNA GFP Target 3
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC57-ITR2-H1-GFP sgRNA Merry Target 1- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210741

    Purpose

    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 1

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Merry sgRNA GFP Target 1
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXPR_003_sgTFAP4 guide 2

    Plasmid

    #193587

    Purpose

    TFAP4 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgTFAP4 guide 2 (TFAP4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LEP-shTAF4.2818

    Plasmid

    #105563

    Purpose

    retrovirally express TAF4 shRNA with puro resistance and GFP marker

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF4 shRNA
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJH4542

    Plasmid

    #200779

    Purpose

    Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    GCaMP6
    

    Use

    Tags

    mNeptune

    Expression

    Worm

    Mutation

    Promoter

    Ptwk-40

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. p CIneo-RL-Let7-3xBulgeB-mut

    Plasmid

    #115369

    Purpose

    Expression vector to produce humanized Renilla luciferase with three mutated (seed-sequence mutations) partially complementary binding sites for human let-7 miRNA in the 3'UTR

    Depositor

    Ramesh Pillai, Witold Filipowicz

    Article

    Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

    Insert

    Renilla Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJH2523

    Plasmid

    #191358

    Purpose

    Punc-25-GCaMP3::UrSL2 ::wCherry unc-54 3' UTR C.elegans DD neurou expression of GCaMP3 RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GCaMP3
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Punc-25

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJH4260

    Plasmid

    #191678

    Purpose

    Ptwk-40s-GCaMP::wScarlet unc-54 3' UTR C.elegans AVA/other neurons expression GCaMP6s wScarlet

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GCaMP6s
    

    Use

    Tags

    wScarlet

    Expression

    Worm

    Mutation

    Promoter

    Ptwk-40

    Available since

    Nov. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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