We narrowed to 1,118 results for: gibson assembly
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Plasmid#202142PurposeLevel 0 plasmid containing an eCFP fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInserteCFP gene
UseSynthetic BiologyMutationNoneAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-roGFP
Plasmid#202140PurposeLevel 0 plasmid containing a roGFP fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInsertroGFP gene
UseSynthetic BiologyMutationNoneAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-eYFP
Plasmid#202139PurposeLevel 0 plasmid containing an eYFP fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInserteYFP gene
UseSynthetic BiologyMutationNoneAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-GUS
Plasmid#202137PurposeLevel 0 plasmid containing a beta glucuronidase reporter gene with CD overhangs used to build a level 1 construct.DepositorInsertBeta glucuronidase gene
UseSynthetic BiologyMutationNoneAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AF-spacer
Plasmid#202130PurposeLevel 0 plasmid containing a small non-coding spacer sequence with AF overhangs used to build level 1 constructs.DepositorInsertSpacer
UseSynthetic BiologyMutationNoneAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5/FRT/TO - RBM28 E5 GT>A minigene
Plasmid#169270PurposeMammalian vector for constitutive expression of RBM28 Exon 5 GT>A minigeneDepositorInsertRBM28 E5 GT>A minigene (RBM28 Human)
ExpressionMammalianMutationNM_018077.2:c.(541+1_541+2delinsA)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO - RBM28 E8 WT minigene
Plasmid#169271PurposeMammalian vector for constitutive expression of RBM28 Exon 8 WT minigeneDepositorAvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtAsmbl
Plasmid#253865PurposeExpression of tobacco Rubisco chaperonin and assembly chaperones in E. coliDepositorInsertsNtRaf1
NtRaf2
NtRbcX
NtBSD2
NtCpn60α
NtCpn60β
NtCpn20
UseSynthetic BiologyExpressionBacterialPromoterT7Available sinceSept. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
MZB184
Plasmid#228488PurposeEmpty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expressionDepositorTypeEmpty backboneTags10xHis-SUMO(Smt3)ExpressionBacterialPromoterT7 PromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
C95m
Plasmid#121001PurposeMoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.DepositorInsertT7 RNA polymerase (Andy please check!)
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C77m
Plasmid#120989PurposeMoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.DepositorInsertAmpR
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C78m
Plasmid#120990PurposeMoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.DepositorInsertChlorR
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C32m
Plasmid#120967PurposeMoClo golden gate assembly CD part for CinR (Quorum-sensing receptor for 3OC14). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohCD carb CinR
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C36m
Plasmid#120970PurposeMoClo golden gate assembly CD part for RhlI (quorum-sensing synthase for 3OC14). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohCD carb RhlI
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C40m
Plasmid#120973PurposeMoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertBlank (Random 50bp sequence)
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C20m
Plasmid#120961PurposeMoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP with ssrA degradation tag; variant of https://www.fpbase.org/protein/yfp/)DepositorInsertsfYFP-ssrA (has BbsI site)
UseSynthetic BiologyTagsssrA degradation tagAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKG1313_pPV3-WPRE
Plasmid#239703PurposeBsaI Golden Gate assembly plasmid encoding the WPRE 3' UTR sequenceDepositorInsertWPRE 3' UTR
UseSynthetic BiologyAvailable sinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH516
Plasmid#102669PurposeConstitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
MZB223
Plasmid#228491PurposeEmpty C-terminal 8xHis tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expressionDepositorTypeEmpty backboneTags8xHisExpressionBacterialPromoterT7 PromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB116
Plasmid#228480Purpose"Empty" C-terminal self-cleaving CPD-tag vector for rapid cloning/DNA Assembly via SwaI restriction enzyme site (bacterial expression)DepositorTypeEmpty backboneTagsCPD-10xHisExpressionBacterialPromoterT7 PromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only