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Plasmid#217533PurposeGenetically encoded fluorescent probe for labelling endogenous excitatory synapses on PV interneurons in vivoDepositorArticleInsertZnF-FingR-PSD95-mGreenLantern (Dlg4 Mouse)
UseAAVTagsmGreenLanternExpressionMammalianMutationPromoterS5E2 enhancerAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBLADE(FP6**)-mCherry
Plasmid#168049PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.DepositorInsertsBlue light-inducible AraC dimers in Escherichia coli
mCherry
UseTagsExpressionBacterialMutationPromoterJ23101** and PBADAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-tagRFP-FLAG-cGAS
Plasmid#86676PurposeExpresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInserttagRFP-FLAG-cGAS
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBLADE(FP6*)-mCherry
Plasmid#168048PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.DepositorInsertsBlue light-inducible AraC dimers in Escherichia coli
mCherry
UseTagsExpressionBacterialMutationPromoterJ23101* and PBADAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS
Plasmid#86677PurposeExpresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.DepositorInsertEGFP-NLS
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG/hTAZ-FL
Plasmid#139979PurposeExpresses full length human Tafazzin with codon modified for cloning. Transgene flanked by AAV2 ITRDepositorInsertFull length human Tafazzin (TAFAZZIN Human)
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEIAV-DIO-TLoop-CHR2YFP
Plasmid#66798Purposeexpression of channelrhodopsin-2-YFP, a light-gated cation channel that can depolarize cells via sodium influx when blue light changes its conformation, using a DIO-based TLoop EIAV vectorDepositorInsertFloxed Chr2-YFP
UseLentiviralTagscitrineExpressionMammalianMutationH134RPromoterCMVTetO7 PromoterAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TetON-Bmpr1A-ca
Plasmid#217536PurposeAAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptorDepositorArticleInsertconstitutively active BMP receptor 1a (BMPR1A Human)
UseAAVTagsHAExpressionMammalianMutationPromoterTet operon (TetO)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV CaMKIIa iChloC 2A tDimer
Plasmid#66709PurposeChloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent proteinDepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVTagsExpressionMammalianMutationE83Q,E90R,E101S,D156N,T159CPromoterCaMKIIaAvailable sinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
KRas FAK biosensor
Plasmid#78301PurposeFRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cellsDepositorInsertKRas FAK biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D
Plasmid#52521PurposeCo-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virusDepositorInsertChR2-2a-hM4D
UseAAV and Cre/LoxTags2xHA on ChR2ExpressionMutationPromoterCBAAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 TIM8,13
Plasmid#170282PurposeExpresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertyeast Tim8 and Tim13 (TIM8 Budding Yeast)
UseTagsTEV-cleavable N-terminal His tag on Tim13 (on bac…ExpressionBacterialMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)
Plasmid#72888Purposefusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, Channelrhodopsin-2, EYFP
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn ChR E90R-D156N-T159C 2A tDimer
Plasmid#52494PurposeHistorical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)DepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVTagsExpressionMammalianMutationE90R, D156N, T159CPromoterhuman synapsin-1 and ribosomal skip sequenceAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:ChR2(H134R)-tdTomato
Plasmid#124237PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomatoDepositorInsertChR2(H134R)-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR
Plasmid#32483PurposeCre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP markerDepositorInsertChR2HA-2a-PSAML141F,Y115F:GlyR
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsChR2 2AExpressionMutationPromoterCAGAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 ChR2 ET-TC 2A tDimer
Plasmid#101361PurposeHigh efficiency channelrhodopsin, neuron specific promoter, red fluorescent proteinDepositorInsertsChannelrhodopsin-2
synaptophysin-red fluorescent protein
UseAAVTagsExpressionMammalianMutationE123T , T159CPromoterhuman SynapsinAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgKEAP1-2
Plasmid#74986PurposesgKEAP1-2, puromycin selectionDepositorInsertKEAP1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn ChR E90R-T159C 2A tDimer
Plasmid#52495PurposeHistorical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)DepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVTagsExpressionMammalianMutationE90R, T159CPromoterhuman synapsin-1 and ribosomal skip sequenceAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn_CapChR1_eYFP
Plasmid#188033PurposeExpresses CapChR1-eYFP in mammalian neuronsDepositorInsertCapChR1-eYFP
UseAAVTagseYFPExpressionMutationS63D, L132C, T159C and N258E mutations in CrChR2PromoterhSynAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only