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B-gTEMP/pcDNA3

Plasmid

#188447

Purpose

B-gTEMP for mammalian expression

Depositor

Takeharu Nagai

Article

Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

Insert

B-gTEMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA Scramble

Plasmid

#162011

Purpose

Lentiviral negative control vector containing scramble shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

scramble shRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psfaMs-Tn5

Plasmid

#183637

Purpose

Expresses anti-mouse nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Article

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti mouse secondary nanobody fused to Tn5

Use

Tags

anti-mouse nanobody

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pET-Neq2X7

Plasmid

#182366

Purpose

Expresses Neq2X7 DNA polymerase

Depositor

Morten Norholm

Article

Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

Insert

Neq2X7 DNA polymerase

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pET-Neq2X

Plasmid

#182365

Purpose

Expresses Neq2X DNA polymerase

Depositor

Morten Norholm

Article

Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

Insert

Neq2X

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-VARNAM

Plasmid

#115552

Purpose

Red fluorescent voltage indicator for mammalian expression

Depositor

Vincent Pieribone

Article

Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

Insert

VARNAM

Use

Tags

Golgi and ER export signal

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LplA(W37V) Coumarin PRIME ligase

Plasmid

#25845

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA-W37V

Use

Tags

Flag and His

Expression

Mammalian

Mutation

W37V

Promoter

Available since

July 27, 2010

Availability

Academic Institutions and Nonprofits only

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(365) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4)

Plasmid

#168188

Purpose

HA-tagged Ubc6E nanobody fused to a truncated OGT of 4 TPRs

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-nUbc6E-(EAAAK)4-OGT(4)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA PTEN KD2

Plasmid

#162009

Purpose

PTEN shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PTEN (PTEN Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA PIK3C2G KD1

Plasmid

#162008

Purpose

PIK3C2gamma shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2G (PIK3C2G Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA SNX9 KD1

Plasmid

#162014

Purpose

SNX9 shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

SNX9 (SNX9 Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA SNX9 KD2

Plasmid

#162015

Purpose

SNX9 shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

SNX9 (SNX9 Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA PIK3C2A KD5

Plasmid

#162006

Purpose

PIK3C2alpha shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2A (PIK3C2A Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA PTEN KD4

Plasmid

#162010

Purpose

PTEN shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PTEN (PTEN Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pVP2del50

Plasmid

#125185

Purpose

Expresses the VP2del50 truncation mutant protein derived from AAV2 VP2

Depositor

Junghae Suh

Article

Thadani et al ACS Nano. 2018 Feb 27;12(2):1445-1454. d

Insert

hexahistidine tag

Use

Tags

Expression

Mammalian

Mutation

Inserted start codon and hexahistidine tag at 2697

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pVP2del30

Plasmid

#125184

Purpose

Expresses the VP2del30 truncation mutant protein derived from AAV2 VP2

Depositor

Junghae Suh

Article

Thadani et al ACS Nano. 2018 Feb 27;12(2):1445-1454. d

Insert

hexahistidine tag

Use

Tags

Expression

Mammalian

Mutation

Inserted start codon and hexahistidine tag at 2637

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)::NL

Plasmid

#141289

Purpose

NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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mtsCOXVIIIx2-B-gTEMP/pcDNA3

Plasmid

#199806

Purpose

Fluorescent temperature indicator B-gTEMP fused with mitochondrial targeting sequence

Depositor

Takeharu Nagai

Article

Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

Insert

B-gTEMP

Use

Tags

mtsCOXVIIIx2

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEHlyA5

Plasmid

#168298

Purpose

Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

Depositor

Luis Ángel Fernández

Article

Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

Insert

N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA

Use

Tags

E-tag, HA-tag, His-tag, Prescission site, and VHH…

Expression

Bacterial

Mutation

Promoter

LacI-Plac

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-VARNAM

Plasmid

#115554

Purpose

Red fluorescent voltage indicator for neuronal expression

Depositor

Vincent Pieribone

Article

Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

Insert

VARNAM

Use

AAV

Tags

Golgi and ER export signal

Expression

Mutation

Promoter

human Synapsin

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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