We narrowed to 5,654 results for: pCas

1. pCAD108_Arg273His_pCMV6Entry-hIRG1

   Plasmid

   #124878

   Purpose

   Expresses hCAD Arg273His mutant in mammalian cells

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Use

   Tags

   myc and FLAG tags

   Expression

   Mammalian

   Mutation

   mutated Arg273 to His

   Promoter

   CMV

   Available since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAFNF-Rac1

   Plasmid

   #163607

   Purpose

   Expresses Rac1 Flp-dependently in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Topo_SpCas9.tracr_LbCas12a.DR

   Plasmid

   #155049

   Purpose

   TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-hHDAC4

   Plasmid

   #21096

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   Histone Deacetylase 4 (HDAC4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   shRNA target sequence was mutated (mutated nucleo…

   Promoter

   CAG

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pCAG-ERas-IH

   Plasmid

   #15688

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

   Insert

   ERas (Eras Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pCAGGS-S-P1-FLAG

   Plasmid

   #177095

   Purpose

   P1 variant S-protein with c-terminal FLAG tag

   Depositor

   Daniel Conway

   Insert

   S-protein P1 (S SARS-CoV-2)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCAG-nirButterfly

   Plasmid

   #136590

   Purpose

   Expression of a genetically-encoded voltage indicator nirButterfly

   Depositor

   Vladislav Verkhusha

   Article

   Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

   Insert

   nirButterfly
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-p21_T145A

   Plasmid

   #40203

   Depositor

   Philipp Mergenthaler

   Article

   Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

   Insert

   p21 (CDKN1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T145A

   Promoter

   CAG

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA1.332 pDUOTK1-L1

   Plasmid

   #162351

   Purpose

   Level 0 terminator acceptor containing dual fluorophores for testing transcription terminator efficiency

   Depositor

   Alistair McCormick

   Article

   Gale et al Front Microbiol. 2021 Jan 15;11:624011.

   Inserts

   eYFP
   LacZ alpha
   mTagBFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. KA2706_eSpCas9-PGKPacdtkCh

    Plasmid

    #124204

    Purpose

    Expression of eSpCas9(1.1) and sgRNA

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-Hoxc4

    Plasmid

    #45550

    Depositor

    Jeremy Dasen

    Article

    Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

    Insert

    Hoxc4 (Hoxc4 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV / b-actin

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCAG-p21_T145D

    Plasmid

    #40204

    Depositor

    Philipp Mergenthaler

    Article

    Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

    Insert

    p21 (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T145D

    Promoter

    CAG

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAMTA2-donor

    Plasmid

    #132501

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    CAMTA2 (CAMTA2 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    none

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAH-MIBP16

    Plasmid

    #51870

    Purpose

    Expresses C-terminal HA-tagged full length rat MIBP1 (cloned into pCAGGS)

    Depositor

    Tomoko Tahira, Kenshi Hayashi

    Article

    Iwashita et al J Biol Chem. 2012 Mar 23;287(13):9887-90

    Insert

    MIBP1 (Hivep2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCAGGS_m-AKAR5’

    Plasmid

    #114140

    Purpose

    Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    AKAR5' C-terminally tagged by KRas C tail
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCALNL_chr13_102010574-102010650

    Plasmid

    #81213

    Purpose

    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 13 (numbers in common name refers to pos. in hg38)

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    gix-Neo-gix deletion cassette upstream of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCBa

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAD96_hIRG1_4-461_pvp008

    Plasmid

    #124874

    Purpose

    Expresses hCAD Asn152Ser mutant in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    mutated Asn152 to Ser, deleted amino acids 1-3 an…

    Promoter

    T7

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCALNL_Ch12_62418577-62418652

    Plasmid

    #81212

    Purpose

    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 12 (numbers in common name refer to pos. in hg38)

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    gix-Neo-gix deletion cassette upstream of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCBa

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Gli3ZF in AINGpCAGGS

    Plasmid

    #121972

    Purpose

    Gli3 zinc fingers

    Depositor

    James Briscoe

    Insert

    Gli3ZF (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc

    Expression

    Mammalian

    Mutation

    N terminus and C terminus deletion only nt 1411-1…

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-EHC

    Plasmid

    #112619

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Emerald (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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