We narrowed to 18,937 results for: Tec

1. shMEK1/2 puro

   Plasmid

   #72567

   Purpose

   Hairpin targeting MEK1 and MEK2 (murine).

   Depositor

   Kevin Janes

   Article

   Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

   Insert

   MEK1/2 (Map2k1, Map2k2 Mouse)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. miniABEmax (pRZ900)

   Plasmid

   #131311

   Purpose

   CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. SFFV-DTAG-CTERM-GFP

   Plasmid

   #185765

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, dTAG

   Mutation

   none

   Promoter

   SFFV

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-GRAB_NEmut

   Plasmid

   #123310

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

   Has Service

   AAV Retrograde and AAV9

   Insert

   GPCR activation based NE control sensor GRAB_NEmut
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-His6MBP-A3A-Gly6His6

   Plasmid

   #187822

   Purpose

   Expression human APOBEC3A in E.coli.

   Depositor

   Hao Wu

   Article

   Fabyanic et al Nat Biotechnol. 2023 Aug 28. doi: 10.103

   Insert

   APOBEC3A (APOBEC3A Human)
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7 promoter (on the backbone)

   Available Since

   July 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUCmini-iCAP-PHP.B3

   Plasmid

   #103004

   Purpose

   non-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

   Insert

   Synthetic construct isolate AAV-PHP.B3 VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. ABE8.13-m

   Plasmid

   #136296

   Purpose

   expresses ABE8.13-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.13-m
   

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUCmini-iCAP-PHP.B2

   Plasmid

   #103003

   Purpose

   non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

   Insert

   Synthetic construct isolate AAV-PHP.B2 VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   Dec. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpCas9-NG-APOBEC-nCas9-Ung

   Plasmid

   #198539

   Purpose

   Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-APOBEC-nCas9-Ung
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. shMEK1/2 v2 puro

    Plasmid

    #72568

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_eA3A-NG-nCas9

    Plasmid

    #163536

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGFP-HBO1-G485A pc2201

    Plasmid

    #167570

    Purpose

    The human HBO1 is fused to C-terminal GFP of EGFP-C1 (Clontech)

    Depositor

    Cristina Cardoso

    Article

    Heinz et al Epigenetics Chromatin. 2019 Mar 14;12(1)

    Insert

    histone acetyltransferase 1 (KAT7 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G485A

    Promoter

    CMV

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti PA-mCit

    Plasmid

    #113451

    Purpose

    Lentiviral ProteinA-mCitrine expression vector

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-mCitrine
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hUBbC

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-eGFP-mPlum-HA

    Plasmid

    #200009

    Purpose

    Donor vector with double fluorescence system for targeted integration using CRISPR/Cas9. Expresses 5´HA--eGFP&PuroR--3´HA--mPlum. Distinguish targeted from random integration by fluorescence.

    Depositor

    Thomas Noll

    Article

    Hertel et al Front Bioeng Biotechnol. 2022 Oct 13;10:

    Insert

    eGFP, mPlum
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PGK-SMASH-NTERM-GFP

    Plasmid

    #185771

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, SMASh

    Mutation

    none

    Promoter

    PGK

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. ABE8.20-d

    Plasmid

    #136301

    Purpose

    expresses ABE8.20-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.20-d
    

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET30a-His6-LOXCATmut

    Plasmid

    #140085

    Purpose

    A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

    Plasmid

    #138146

    Purpose

    Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PspCas13b
    

    Use

    Lentiviral

    Tags

    NES

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. A*02:02:01:01

    Plasmid

    #203277

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*02:02:01:01 (HLA-A Human)
    

    Expression

    Mammalian

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMJA285= pSin-TCRab-CMVp-Puro

    Plasmid

    #116875

    Purpose

    stable TCR expression in human T cells

    Depositor

    Marcos Alcocer

    Article

    Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

    Insert

    TCR (TRAV10, TRBV25-1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    deletion of EF1-a promotor

    Promoter

    CMV Promotor

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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