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AAV-nEF-dCas9

Plasmid

#106430

Purpose

Expresses SpdCas9 from the nEF promoter in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

nEF-dCas9

Use

AAV and CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Promoter

nEF

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pG2J

Plasmid

#102882

Purpose

Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Inserts

OsTIR1
mGFP-AID

Tags

AID(71-114)

Expression

Yeast

Promoter

ADH1 and TEF1

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-ArchT-GFP

Plasmid

#28307

Purpose

Cre-dependent viral expression of ArchT-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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GFP-Epac1 SAAX

Plasmid

#118313

Purpose

Expression of human Epac1

Depositor

Philip Stork

Article

Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

Insert

Epac1 SAAX (RAPGEF3 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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ABE8.13-m

Plasmid

#136296

Purpose

expresses ABE8.13-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.13-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-hSOX10

Plasmid

#24749

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Mutation

wildtype sequence

Available Since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
MSCV N-term HA-FLAG puro BRD7

Plasmid

#41934

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

HA-Flag

Expression

Mammalian

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-OSF-KATNA1

Plasmid

#160051

Purpose

Mammalian expression vector for KATNA1 (KATANIN-P60) with N-terminal One-Strep-Flag tag. Internal ID: WISP20-18.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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5HT2C-RNA2-Flag

Plasmid

#79679

Purpose

serotonin receptor 2C isoform 2 with C-terminal Flag tag, non-edited isoform

Depositor

Stefan Stamm

Article

Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

Insert

serotonin receptor 2C, RNA2 (HTR2C Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only
FASTHDR-Cterm-mTagBFP2-Blasticidin

Plasmid

#167207

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mTagBFP2

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-MPRA-MluI-SpeI-EcoRI

Plasmid

#190196

Purpose

Empty vector for inserting MPRA libraries into AAV

Depositor

Hyejung Won

Article

McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

Type

Empty backbone

Use

AAV; Massively parallel reporter assay (mpra)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMCS.DT-A

Plasmid

#22730

Depositor

Mark Magnuson

Article

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

DT-A

Use

Bac recombineering

Mutation

This is a gap-repair vector designed for retrievi…

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_1_001

Plasmid

#167347

Purpose

ddPCR gating control for droplets containing all 5 targets (2 in pol)

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat_env

Use

Other

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-dGFP-JARID1C

Plasmid

#74776

Purpose

retroviral expression of JARID1C

Depositor

Giovanni Tonon

Article

Rondinelli et al Nucleic Acids Res. 2015 Mar 11;43(5):256

Insert

JARID1C (KDM5C Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only
pSIN-Ldha

Plasmid

#175939

Purpose

knockout mouse Ldha

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Ldha gRNA (Ldha Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FLCN

Plasmid

#72290

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pBactin-Tubg1-mEGFP

Plasmid

#196870

Purpose

Expression of the gamma-tubulin fused to enhanced (E)-GFP

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Tubg1-mEGFP (Tubg1 Mouse)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_14_004

Plasmid

#167350

Purpose

ddPCR gating control for droplets containing the env target

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

env

Use

Other

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_32_007

Plasmid

#167353

Purpose

ddPCR gating control for droplets containing 5'pol or 3'pol targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol

Use

Other

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_17_005

Plasmid

#167351

Purpose

ddPCR gating control for droplets containing gat and tat targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gat_tat

Use

Other

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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