We narrowed to 15,990 results for: nes
-
Plasmid#138525PurposeExpresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.DepositorInsertControl non-PRC2 binding A-tract repeat RNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mouse 5' Npm1 AID GFP PuroR
Plasmid#127899PurposeHDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Npm1 GeneDepositorAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
MAP2K3_pLX307
Plasmid#98355PurposeLentiviral expression of MAP2K3DepositorInsertMAP2K3 (MAP2K3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationQ85KPromoterE1FaAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-Rab8a-TN
Plasmid#86080PurposeA bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.DepositorInserthRab8A (RAB8A Human)
TagsGSTExpressionBacterialMutationChanged threonine 22 to asparginePromotertacAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
CΔ1
Plasmid#74296PurposeMammalian expression of treacle (residues 1176-1302) fused to GFPDepositorInserttreacle ribosome biogenesis factor 1 (Tcof1 Mouse)
TagsGFPExpressionMammalianMutationExpresses residues 1176 - 1302PromoterCMVAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K24,83,88,104A
Plasmid#58408PurposeExpresses murine IFITM3-K24,83,88,104A with an N-terminal HA tag in mammalian cells (Lysine-less nonubiquitinated mutant)DepositorInsertIFITM3 (Ifitm3 Mouse)
TagsHAExpressionMammalianMutationLysines 24, 83, 88, and 104 to AlaninePromoterCMV IEAvailable SinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBT310_pBS-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
Plasmid#52543PurposeExpression of FRT5-flanked Neo; homologous recombination targeting vector cloningDepositorInsertsPhiC31o attP (full length)
Neo
PhiC31o integrase
ExpressionMammalianAvailable SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Map7-Start-1351
Plasmid#46075DepositorAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
HOXC5 (human) HIS-tag pET
Plasmid#8563DepositorInsertHOXC5 (HOXC5 Human)
TagsHis and T7ExpressionBacterialMutationStarts at Amino Acid #2 (Ser); makes protein by W…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
EFS-SpdCas9-Dnmt3A/3L-V3
Plasmid#222500PurposeExpresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherryDepositorInsertS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; R887E and E814…PromoterEFSAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
EFS-SpdCas9-Dnmt3A/3L-catΔ
Plasmid#222501PurposeExpresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) followed by P2A-mCherryDepositorInsertS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; C706A and R832…PromoterEFSAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLT3-D835Y-V5/HIS
Plasmid#236007Purposeexpression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic LeukemiaDepositorInserthuman FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)
TagsV5/HisExpressionMammalianMutationD835Y substitutionPromoterCMVAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLPC-dnMAML1-mVenus
Plasmid#171023PurposeRetroviral vector for CMV promoter driven expression of dominant negative (DN) MAML1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).DepositorInsertMAML1 Dominant Negative (MAML1 Human)
UseRetroviralTagsmVenusExpressionMammalianMutationamino acids 12-74 only, hence conferring dominant…PromoterCMVAvailable SinceApril 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-3xFlag-dCas13-mTET2HxDCD
Plasmid#228235PurposeFor targeted tethering of an inactive mutant (HxD) of the catalytic domain of mouse TET2 using dCas13DepositorInsertsUseCRISPRExpressionMammalianMutationCD (cysteine-rich, dioxygenase domain) truncation…PromoterCAGAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP13999 - pAAV-AiE0387m-minBG-iCre(R297T)-BGHpA (Alias: CN3999)
Plasmid#208166PurposeAiE0387m is an enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytesDepositorInsertiCreR297T
UseAAVMutationNAPromoterBeta Globin minimal promoterAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
AiP14253 - pAAV-AiE0380h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4253)
Plasmid#208165PurposeAiE0380h_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytesDepositorInsertSYFP2
UseAAVMutationNAPromoterBeta Globin minimal promoterAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
AiP12089 - pAAV-AiE0390m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2089)
Plasmid#208134PurposeDirect-expressing SYFP2 in astrocytes AAV VirusDepositorInsertSYFP2
UseAAVMutationNAPromoterBeta Globin minimal promoterAvailable SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-NLS-PTEN
Plasmid#206984PurposeLentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLSDepositorAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPyPGK-HA-EGFP-Myc-TM-IRES-Pac
Plasmid#183606PurposeMammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.DepositorInsertEGFP-TM
TagsHA, Human PDGFRB transmembrane domain, Mouse IgGK…ExpressionMammalianPromoterMouse Pgk1Available SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-hRedOpsin-TGFB2
Plasmid#164428PurposeAAV plasmid expressing TGFB2 in photoreceptorsDepositorAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only