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Mito-mhYFP

Plasmid

#186526

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to mitochondria

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

Human COX8a first 29 amino acids

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET-11-LECT2-V40

Plasmid

#183031

Purpose

Expression of LECT2 V40 variant protein from T7 promoter

Depositor

Gareth Morgan

Article

Belonogov et al bioRxiv 2022.03.01.482540

Insert

LECT2 (LECT2 Human)

Expression

Bacterial

Mutation

V40 variant

Promoter

T7

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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BSA1_His

Plasmid

#202766

Purpose

bovine serum albumin design BSA1, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

BSA1

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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p006-Banana-Late

Plasmid

#112251

Purpose

ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

ATF1 under osmY promoter

Expression

Bacterial

Promoter

osmY

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLEW100cre-EP1

Plasmid

#33345

Purpose

For expression of tetracycline-inducible CRE in Trypanosoma brucei

Depositor

George Cross

Article

Scahill et al Mol Biochem Parasitol. 2008 Jan;157(1):7

Insert

cre recombinase

Use

Cre/Lox; Trypanosoma brucei expression vector

Promoter

TET operator

Available Since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only

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pMC-1-AtUbi10-6

Plasmid

#197700

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AtUbi10 promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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Clathrin-mhYFP

Plasmid

#186527

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to clathrin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

Clathrin

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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p5E-mnx1

Plasmid

#82402

Purpose

Gateway-compatible zebrafish mnx1 promoter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mnx1 promoter

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-MinE-His

Plasmid

#133623

Purpose

Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

Depositor

Petra Schwille

Article

Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

Insert

MinE-His (minE E. coli)

Tags

His

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGY208

Plasmid

#239803

Purpose

Express mCherry in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

mCherry

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBabe-Hygro-GFP

Plasmid

#61215

Purpose

Retrovirus expressing GFP

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

GFP

Use

Retroviral

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-dCas9

Plasmid

#218371

Purpose

Expression of dCas9 for CRISPR-interference

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Promoter

PtetA

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINS-UBE3C

Plasmid

#66711

Purpose

Expresses UBE3C HECT domain in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

KiAA0010 (UBE3C )

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
pSB1C3-Lux

Plasmid

#109383

Purpose

Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-LUX operon

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT12.G

Plasmid

#201000

Purpose

pLv1-pUC18R6KT-miniTn7-Km-sfGFP, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-9-35S-11

Plasmid

#197759

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

35S terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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yWL229 MCP-GFP-eRF3

Plasmid

#197053

Purpose

pUBC-NLS-HA-2xMCP-1xGFP-eRF3

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-GFP-eRF3

Use

Lentiviral

Expression

Mammalian

Promoter

ubc

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Ptac

Plasmid

#218364

Purpose

Empty backbone for Tn7-site insertion with lacIq-Ptac expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pTRANS_250d

Plasmid

#91124

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

PvUbi2

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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YN2_1_LT58_X2site

Plasmid

#177705

Purpose

Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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