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pT7C-DNAJB6

Plasmid

#177657

Purpose

Recombinant protein synthesis of human DNAJB6

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

DNAJB6 (DNAJB6 Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-AviMta3-3xFiBsd

Plasmid

#140962

Purpose

full-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Article

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis associated 3 (Mta3 Synthetic, Mouse)

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgAR3

Plasmid

#167002

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 3 (AR Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgUpf1

Plasmid

#161810

Purpose

guide RNA for Upf1

Depositor

Yi Zhang

Article

Fu et al Sci Adv. 2020 May 29;6(22):eaay5181. doi

Insert

sgRNA targeting mouse Upf1 (Upf1 Synthetic, Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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ELF3_pet28a

Plasmid

#131658

Purpose

Bacterial expression of ETS gene ELF3

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF3 (ELF3 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-mAV

Plasmid

#129472

Purpose

Mammalian expression of full-length mouse archvillin (Supervillin, isoform 2)

Depositor

Elizabeth Luna

Article

Oh et al J Cell Sci. 2003 Jun 1. 116(Pt 11):2261-

Insert

Supervillin, isoform 2 (Svil Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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KN206: pMVP/eCFP-DEST

Plasmid

#121854

Purpose

pMVP destination vector, empty expression vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JN607: pMVP/SB-DEST

Plasmid

#121860

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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FB005

Plasmid

#119678

Purpose

Coding sequence nptII from E. coli domesticated into pUPD2 with AATG/GCTT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

geneticin resistance marker

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pRS315-48nt-telo-I-SceI

Plasmid

#67623

Purpose

telomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeats

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

3' gal1 unique sequence
ura4 (ura4 Fission Yeast)
48 bp telomere repeats
hph

Use

Yeast cen/ars plasmid

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) FITM2

Plasmid

#107504

Purpose

Expresses FITM2, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

FITM2 (codon optimized)

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized sequence

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-BASIC- ARHGAP29 Mutant PROMOTER

Plasmid

#104156

Purpose

luciferase reporter with ARHGAP29 mutant promoter

Depositor

Marius Sudol

Article

Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

Insert

ARHGAP29 promoter (ARHGAP29 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

delete two TEAD binding sites

Promoter

ARHGAP29

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA-RagC(L91P)

Plasmid

#99647

Purpose

Bacterial expression of RagA-RagC(L91P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

L91P

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMyc-C1-Cep123 (isoform 2)

Plasmid

#73327

Purpose

Encodes isoform 2 of Cep123 (Cep89/CCDC123) fused to a myc tag

Depositor

Michel Bornens

Article

Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

Insert

Cep123 (CEP89 Human)

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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GST-Delta23C11orf83

Plasmid

#65849

Purpose

bacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

UQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion of the 23 first aa (TM)

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hmga2 ATG wt

Plasmid

#52034

Purpose

encodes wt HMGA2

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Expression

Mammalian

Mutation

starting ATG deleted

Promoter

CMV

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
psbA2-PHLS (b)

Plasmid

#52307

Purpose

Replaces the psbA2 gene in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Promoter

psbA2

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-GST-Presc-mAkt1 (S473T)

Plasmid

#48806

Purpose

expresses mutant Akt1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

Akt1 (Akt1 Mouse)

Tags

HA-GST-Presc

Expression

Mammalian

Mutation

S473T

Promoter

CMV

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pmalc4x_I499F_TEV

Plasmid

#47703

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499F

Depositor

Raquel Lieberman

Article

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Olfactomedin-like domain with I499F mutation (MYOC Human)

Tags

MBP

Expression

Bacterial

Mutation

changed I499F

Promoter

Ptac

Available Since

Sept. 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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Δconst-GFP

Plasmid

#33114

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the C-terminal constant region (amino…

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only