We narrowed to 13,341 results for: GAN

1. pAAV.CMV.RGS4.C-HA

   Plasmid

   #154847

   Purpose

   Expresses murine RGS4 protein with HA-tag at C-terminus

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Regulator of G protein Signaling 4 (Rgs4 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

   Plasmid

   #115789

   Purpose

   Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   NDP (NDP Human)
   

   Use

   Tags

   alkaline phosphatase and flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH0103_LASP1_LIM

   Plasmid

   #109780

   Purpose

   Protein expression and purification of LASP1_LIM

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   LASP1_LIM (LASP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME GFP-PCNA

   Plasmid

   #105977

   Purpose

   entry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNA

   Depositor

   Caren Norden

   Article

   Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

   Insert

   PCNA (PCNA Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b LFn-Fla 3A

   Plasmid

   #84872

   Purpose

   Bacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   Fla
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   3 C-terminal leucines have been replaced by alani…

   Promoter

   T7

   Available since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pUBC_HA_PP7-mVenus

   Plasmid

   #111268

   Purpose

   Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag

   Depositor

   John Rinn

   Article

   Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

   Insert

   PP7-mVenus
   

   Use

   Synthetic Biology

   Tags

   HA, SV40 NLS, and mVenus

   Expression

   Mammalian

   Mutation

   PP7: C68S, S69G [delta]70-75 (non-aggregating)

   Promoter

   hUBC

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. TIMP1-bio-His

   Plasmid

   #52089

   Purpose

   Expresses full-length Metalloproteinase inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TIMP1 (TIMP1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHJ4: LexA-VHH-Caffeine

   Plasmid

   #108242

   Purpose

   LexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.

   Depositor

   Jerome Bonnet

   Article

   Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

   Insert

   LexA DNA binding domain fusing with VHH-Caffeine antibody
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pLacO1

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSF-G1(6CNW)RS

   Plasmid

   #222873

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PhyB-mCherry-CAAX

    Plasmid

    #51567

    Purpose

    Constitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    CAAX and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available since

    Aug. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSL0812 (pCRISPR(BbsI)_Vch)

    Plasmid

    #200898

    Purpose

    Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCRISPR (entry)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-hSyn-FlicR1

    Plasmid

    #74143

    Purpose

    Express FlicR1 preferentially in neurons

    Depositor

    Robert Campbell

    Article

    Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

    Insert

    FlicR1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Empty Target Control

    Plasmid

    #127667

    Purpose

    Encodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. TIMP3-bio-His

    Plasmid

    #53418

    Purpose

    Expresses full-length Metalloproteinase inhibitor 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TIMP3 (TIMP3 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCas9-CAT

    Plasmid

    #80323

    Purpose

    Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Inserts

    Cas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR

    Tags

    3X FLAG and NLS

    Expression

    Mutation

    Promoter

    TgTUB1

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-AR-1-333

    Plasmid

    #104194

    Purpose

    Expresses human AR aa 1-333 in pGEX-5x-1 bacteral expression vector with GST

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    human androgen receptor aa 1-333 (AR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMOD_B2312

    Plasmid

    #91070

    Purpose

    Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    PvUbi1

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2-mRuby-Rab8a

    Plasmid

    #194337

    Purpose

    Expresses mRuby Rab8a in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Insert

    Rab8 (RAB8A Canis lupus)
    

    Use

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX302-JSP1-V5 puro

    Plasmid

    #87773

    Purpose

    Expresses V5 tagged human JSP1 (DUSP22)

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    JSP1 (DUSP22 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C1-hArfaptin1

    Plasmid

    #206404

    Purpose

    Mammalian expression of hArfaptin1

    Depositor

    Kazuhisa Nakayama, Hye-Won Shin

    Article

    Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

    Insert

    Arfaptin1 (ARFIP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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