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NIK1 A11_pECIA14

Plasmid

#114777

Purpose

Prey vector NIK1 A11_pECIA14 should be used with bait vector NIK1 A11_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G16000 (NIK1 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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RKL1 I6_pECIA14

Plasmid

#114781

Purpose

Prey vector RKL1 I6_pECIA14 should be used with bait vector RKL1 I6_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G48480 (RKL1 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT5G16900 O2_pECIA14

Plasmid

#114762

Purpose

Prey vector AT5G16900 O2_pECIA14 should be used with bait vector AT5G16900 O2_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G16900 (AT5G16900 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SERK2 P5_pECIA14

Plasmid

#114768

Purpose

Prey vector SERK2 P5_pECIA14 should be used with bait vector SERK2 P5_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G34210 (SERK2 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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NIK3 nA5_pECIA14

Plasmid

#114769

Purpose

Prey vector NIK3 nA5_pECIA14 should be used with bait vector NIK3 nA5_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G60800 (NIK3 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G49100 L12 _pECIA14

Plasmid

#114740

Purpose

Prey vector AT1G49100 L12 _pECIA14 should be used with bait vector AT1G49100 L12 _pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G49100 (AT1G49100 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-mkgDUX4mal*leu*

Plasmid

#21173

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX4 (DUX4 Human)

Expression

Bacterial and Mammalian

Mutation

First point mutation at nucleotide 5114 (numberin…

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only
NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75233

Purpose

CRISPR/Cas9 plasmid against human NFATc2

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75234

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75235

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75238

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75239

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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T7-5'UTR-SuperDn29-bpNLS-3'UTR-polyA (CJT611)

Plasmid

#248220

Purpose

bpNLS-SuperDn29 IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-SuperDn29-bpNLS-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

Super Dn29(M6I, E70G, A224P, G227V, I233K, K248R,…

Promoter

T7

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-SV40NLS-Stab-exin21-Bxb1(A315R)-Stab (CJT604)

Plasmid

#248210

Purpose

SV40 NLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

SV40NLS-Stab-exin21-Bxb1(A315R)-Stab

Use

CRISPR; In vitro transcription

Tags

SV40NLS

Expression

Mammalian

Mutation

Bxb1(A315R)

Promoter

CMV and T7

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pFBDMeGmTet1CD (pc2838)

Plasmid

#246924

Purpose

Expresses mCherry-tagged catalytic active domain of mouse Tet1 in mammalian cells. N-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Ludwig et al Nucleic Acids Res. 2017 Mar 17;45(5):243

Insert

Tet1 (Tet1 Mouse)

Tags

GFP

Expression

Insect

Mutation

aa1365-2057

Promoter

Polyhedrin Promoter

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pFBDMchmTet1CD (pc2859)

Plasmid

#246925

Purpose

Expresses mCherry-tagged catalytic active domain of mouse Tet1 in mammalian cells. N-terminal tagged to mCherry.

Depositor

Cristina Cardoso

Article

Ludwig et al Nucleic Acids Res. 2017 Mar 17;45(5):243

Insert

Tet1 (Tet1 Mouse)

Tags

mcherry

Expression

Insect

Mutation

aa 1365-2057

Promoter

Polyhedrin Promoter

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pSRG11 (eft-3p::scfv(glo)::gfp(smu-1 introns)::tbb-2 3’UTR)

Plasmid

#245121

Purpose

Optimized SunTag antibody for expression in C. elegans. Useful for visualization of translation of GCN4 encoding mRNAs or for visualization of GCN4-tagged proteins

Depositor

Suzan Ruijtenberg

Article

van der Salm et al Development. 2025 May 15;152(10):dev2044

Inserts

eft-3p
scFv (GLO)
GFP (GLO)
tbb-2 3'UTR
left recombination arm MosSCI ttTi5605
right recombination arm MosSCI ttTi5605

Expression

Bacterial and Worm

Available Since

Jan. 8, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 GST-SHP2 H116A E252A

Plasmid

#244493

Purpose

Bacterial Expression of pH insensitive SHP2 mutant

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Histidine 116 to Alanine and Glutamate 25…

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Neo-2/15-TGFa-SPMn-SpyTag003

Plasmid

#246650

Purpose

Expresses Neo-2/15-TGFa-SPMn-SpyTag003 in mammalian cells for calcium-induced NeissLock protein ligation to epidermal growth factor receptor (EGFR)

Depositor

Mark Howarth

Article

Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021

Insert

Neo-2/15-TGFa-SPMn-SpyTag003

Tags

Signal peptide and SpyTag003

Expression

Mammalian

Mutation

Construct contains the tPA signal peptide, Neo2/…

Promoter

CMV promoter

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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PAX3::FOXO1-2A-sfGFP

Plasmid

#240098

Purpose

human PAX3::FOXO1 in -2A-sfGFP backbone (Plasmid# 74668)

Depositor

Genevieve Kendall

Article

Kucinski et al Cell Rep. 2025 Jul 22;44(7):115923. doi:

Insert

PAX3::FOXO1 (FOXO1, PAX3 Human)

Expression

Mammalian

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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