We narrowed to 15,906 results for: grna

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pHiFi Cas9-2×sgRNA (empty, donor)

Plasmid

#162277

Purpose

All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.

Depositor

Kazuhisa Nakayama

Publication

Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi

Insert

HiFi Cas9

Use

CRISPR

Tags

BPNLS and FLAG tag

Expression

Mammalian

Mutation

SpCas9 (R691A)

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSaCas9-1xms2-2x3’UTR

Plasmid

#122946

Purpose

For expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTR

Depositor

Baisong Lu

Publication

Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

Insert

SaCas9-MS2-HBB 3' UTR

Use

AAV

Expression

Mammalian

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA-SV40-puro

Plasmid

#240539

Purpose

Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Publication

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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U6-(BbsI)sgRNA_CAG-Venus-bpA

Plasmid

#86985

Purpose

For cloning and expression of sgRNA together with Venus expression

Depositor

Ralf Kuehn

Publication

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Venus

Expression

Mammalian

Mutation

Venus GFP variant

Promoter

CAG

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pU6_rd6_pegRNA_(PP7-C4-Q1)

Plasmid

#232433

Purpose

pegRNA with optimized 3' modifications to correct the rd6 mutation

Depositor

Gil Westmeyer

Publication

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pM123: pAAV-EFS-CasRx-control presgRNA

Plasmid

#166871

Purpose

AAV vector for expressing CasRx and control presgRNA for RNA-editing

Depositor

Guei-Sheung Liu

Publication

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Inserts

U6-control presgRNA
RfxCas13d

Use

AAV and CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

EFS and U6

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

Plasmid

#86987

Purpose

For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

Depositor

Ralf Kuehn

Publication

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9

Expression

Mammalian

Promoter

CAG

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

Plasmid

#168290

Purpose

CRISPR-activator Suntag-P65-HSF1 (SPH)

Depositor

Hui Yang

Publication

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

dCas9, Suntag-P65-HSF1

Expression

Mammalian

Promoter

CAG

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLSB-NAT

Plasmid

#166698

Purpose

Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.

Depositor

Robin Allshire

Publication

Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

Inserts

tRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe

Expression

Yeast

Promoter

adh15 promoter and tRNA promoter

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBBR-Cas9ΔyqhD-HA500

Plasmid

#220369

Purpose

A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

Depositor

Yilan Liu

Publication

Wen et al Microb Cell Fact. 2024 Dec 30;23(1):349.

Inserts

SpCas9
sgRNA for yqhD in P. putida KT2440

Use

CRISPR; Broad host range

Expression

Bacterial

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pS448∙CsR

Plasmid

#122096

Purpose

CRISPR/Cas9 counterselection in Gram-negative bacteria

Depositor

Pablo Ivan Nikel

Publication

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Inserts

Cas9
sgRNA

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pem7 and Pm

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pNR4A1.1.0-gDNA

Plasmid

#112391

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1

Depositor

Kevin White

Insert

NR4A1 (NR4A1 Human)

Use

CRISPR

Expression

Mammalian

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pJW1259

Plasmid

#61251

Purpose

Cas9 plasmid with sgRNA deleted; for use in C. elegans

Depositor

Jordan Ward

Publication

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

Deletion

Use

CRISPR

Expression

Worm

Mutation

Deleted sgRNA(F+E) sequence from pJW1219

Available since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHR-UCOE-SFFV-Zim3-dCas9-BFP

Plasmid

#188776

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9-mTagBFP2

Use

Lentiviral

Tags

HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pML104-KanMX-sgRNAv2

Plasmid

#254712

Purpose

Yeast CRISPR-Cas9 backbone with sgRNA cassette for rapid PCR-based guide insertion; KanMX/G418 selection in S. cerevisiae, Amp/Kan propagation in E. coli.

Depositor

Brian Strahl

Publication

Rostamian et al STAR Protoc. 2026 Jul 28;7(3):104751. do

Insert

YKU70 sgRNA (YKU70 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52 promoter

Available since

Aug. 18, 2026

Availability

Academic Institutions and Nonprofits only

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LentiCrispr V2 Mboat2 sgRNA1

Plasmid

#258333

Purpose

Crispr mediated knockout of Mboat2 gene

Depositor

Xiaoai Zhao

Publication

Zhao et al Sci Adv. 2026 Jul 31;12(31):eaeh9771. do

Insert

sgRNA1 targeting Mboat2 (Mboat2 Mouse)

Use

CRISPR and Lentiviral

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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LentiCrispr V2 Elovl5 sgRNA1

Plasmid

#258337

Purpose

Crispr mediated knockout of Elovl5 gene

Depositor

Xiaoai Zhao

Publication

Zhao et al Sci Adv. 2026 Jul 31;12(31):eaeh9771. do

Insert

sgRNA1 targeting Elovl5 (Elovl5 Mouse)

Use

CRISPR and Lentiviral

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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LentiCrispr V2 Pla2g4e sgRNA1

Plasmid

#258363

Purpose

Crispr mediated knockout of Pla2g4e gene

Depositor

Xiaoai Zhao

Publication

Zhao et al Sci Adv. 2026 Jul 31;12(31):eaeh9771. do

Insert

sgRNA1 targeting Pla2g4e (Pla2g4e Mouse)

Use

CRISPR and Lentiviral

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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LentiCrispr V2 Fads2 sgRNA1

Plasmid

#258338

Purpose

Crispr mediated knockout of Fads2 gene

Depositor

Xiaoai Zhao

Publication

Zhao et al Sci Adv. 2026 Jul 31;12(31):eaeh9771. do

Insert

sgRNA1 targeting Fads2 (Fads2 Mouse)

Use

CRISPR and Lentiviral

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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LentiCrispr V2 Agpat3 sgRNA1

Plasmid

#258364

Purpose

Crispr mediated knockout of Agpat3 gene

Depositor

Xiaoai Zhao

Publication

Zhao et al Sci Adv. 2026 Jul 31;12(31):eaeh9771. do

Insert

sgRNA1 targeting Agpat3 (Agpat3 Mouse)

Use

CRISPR and Lentiviral

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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