We narrowed to 23,621 results for: promoter

1. pMD19_STARR-seq_plants

   Plasmid

   #117379

   Purpose

   STARR-seq vector with 35s core promoter for transient expression in plant protoplasts

   Depositor

   Xiaoyu Zhang

   Article

   Ricci et al Nat Plants. 2019 Nov 18. pii: 10.1038/s4

   Type

   Empty backbone

   Tags

   GFP derived from pMDC107

   Expression

   Plant

   Promoter

   35s core promoter

   Available Since

   Nov. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. HA-Rab8a-CA (Q67L)

   Plasmid

   #101050

   Purpose

   Expresses HA-tagged human Rab8-CA (Q67L) in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Article

   Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

   Insert

   Rab8a (RAB8A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Q67L

   Promoter

   CMV

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO-Tet-On shYAP1-3

   Plasmid

   #193666

   Purpose

   Tet inducible knockdown of YAP1

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 6A NLRP1 CARD-FLAG WT

   Plasmid

   #163978

   Purpose

   Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-ZDHHC9-C169S

   Plasmid

   #231673

   Purpose

   Expresses human ZDHHC9-C169S protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC9 (ZDHHC9 Human)
   

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Polymerase-unfused click editor - pCMV-T7-PCV2-nCas9 (LM2702)

   Plasmid

   #208961

   Purpose

   Polymerase-unfused click editor comprised of PCV2 fused to nCas9, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR-XL-rAkap9

   Plasmid

   #196867

   Purpose

   Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akap9 (Akap9 Rat)
   

   Expression

   Bacterial

   Promoter

   Lac promoter and T7 promoter

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2K7bsdUBI-mCherry-STIM1

   Plasmid

   #114178

   Purpose

   Lentiviral vector for expression of mCherry-STIM1

   Depositor

   Nicolas Demaurex

   Article

   Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry (inserted after signal peptide)

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLCA.66/2272

   Plasmid

   #22733

   Depositor

   Mark Magnuson

   Article

   Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

   Insert

   Lox66-pU(delta)TK-EM7Neo-Lox2272
   

   Use

   Cre/Lox; Bac recombineering

   Mutation

   This vector was designed for assembling gene targ…

   Available Since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1+_FH-AGO2-5xA

    Plasmid

    #92007

    Purpose

    Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824A, S828A, T830A, S831A, S834A (5xA)

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-FLAG-mTET2 CD mutant

    Plasmid

    #89896

    Purpose

    Expresses mouse TET2 CD mutant in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    TET2 (Tet2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    CD (cysteine-rich, dioxygenase domain) truncation…

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1+_FH-AGO2-5xE

    Plasmid

    #92008

    Purpose

    Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824E, S828E, T830E, S831E, S834E (5xE)

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX317-VRK1-K179E

    Plasmid

    #199644

    Purpose

    Expresses kinase-inactive VRK1 (K179E)

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Mutation

    K179E

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-HNF1A-WT-Flag-UTR

    Plasmid

    #183237

    Purpose

    Expression of FLAG tagged HNF1A

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    HNF1 homeobox A (HNF1A Human)
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

    Plasmid

    #38262

    Depositor

    Robert Lefkowitz

    Article

    Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

    Insert

    β-arrestin2 (Arrb2 Rat)
    

    Tags

    Flag and PIP5K Iα core kinase domain (residues 18…

    Expression

    Mammalian

    Mutation

    Arg 233, Arg237, and Lys251 converted to Glutamin…

    Promoter

    CMV

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. NSP4-WT-Flag

    Plasmid

    #210339

    Purpose

    Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-TRE-NS1-dTomato

    Plasmid

    #175278

    Purpose

    AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    NS1; dTomato (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Tags

    dTomato (from bidirectional promoter)

    Promoter

    bidirectional TRE promoter

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV-EF1aGFP2AKAT7(E508Q)-W

    Plasmid

    #159293

    Purpose

    Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    KAT7 E508Q
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

    Promoter

    human EF1a promoter

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

    Plasmid

    #159086

    Purpose

    Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.

    Depositor

    Jeremy Day

    Article

    Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589

    Insert

    empty crRNA-acRNA backbone, dCas9-P2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Promoter

    hSYN, U6

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-EGFP MASTL G44S (siRNA resistant)

    Plasmid

    #191012

    Purpose

    Expresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

    Depositor

    Johanna Ivaska

    Article

    Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Microtubule-associated serine/threonine kinase like (MASTL Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    G44S

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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