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Showing: 7901 - 7920 of 8705 results

  1. pEvolvR-mCherry-Hypa-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249076
    Purpose
    Expresses nucleus-localized Hypa-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Hypa-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249076-plasmid-map-sequence-id-496314

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  2. pEvolvR-mCherry-SpRY-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249074
    Purpose
    Expresses nucleus-localized SpRY-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    SpRY-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249074-plasmid-map-sequence-id-496313

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  3. pEvolvR-mCherry-Slug-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249078
    Purpose
    Expresses nucleus-localized Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Slug-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249078-plasmid-map-sequence-id-496311

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  4. pSS2.1-Cas9-HH-gRNADhps-HDV

    Plasmid
    #241015
    Purpose
    Cas9 expression cassette encoding a guide RNA targeting the Dhps locus, flanked by a hammerhead (HH) ribozyme at the 5′ end and a hepatitis delta virus (HDV) ribozyme at the 3′ end
    Depositor
    Alan Smulian
    Article
    Sayson et al mBio. 2025 Sep 23:e0182525. doi: 10.1128
    Inserts
    Cas9-Hammerhead (HH) Ribozyme-gRNA(Dhps)-Hepatitis Delta Virus (HDV) Ribozyme
    blasticidin-S deaminase
    Expression
    Yeast
    Available Since
    Nov. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    241015-plasmid-map-sequence-id-479775

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  10. M7138

    Plasmid
    #219774
    Purpose
    MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of ORCA3 promoter and terminator
    Depositor
    Karen Sarkisyan
    Article
    Balakireva et al bioRxiv 2025.02.17.636591; doi: https://
    Insert
    pNOS-HygR-ocsT | pORCA3-nnLuz-ORCA3_T
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    pNOS-HygR-ocsT | pORCA3-nnLuz-ORCA3_T
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    219774-plasmid-map-sequence-id-437302

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  11. M7691

    Plasmid
    #219775
    Purpose
    MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of WRKY70 promoter and terminator
    Depositor
    Karen Sarkisyan
    Article
    Balakireva et al bioRxiv 2025.02.17.636591; doi: https://
    Insert
    pNOS-HygR-ocsT | pWRKY70-nnLuz-WRKY70_T
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    pNOS-HygR-ocsT | pWRKY70-nnLuz-WRKY70_T
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    219775-plasmid-map-sequence-id-437303

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  12. pNK3558

    Plasmid
    #219776
    Purpose
    MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of p35S promoter and act2 terminator
    Depositor
    Karen Sarkisyan
    Article
    Balakireva et al bioRxiv 2025.02.17.636591; doi: https://
    Insert
    pNOS-HygR-ocsT | p35S-nnLuz-act2
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    pNOS-HygR-ocsT | p35S-nnLuz-act2
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    219776-plasmid-map-sequence-id-438202

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  19. pCas3-APOBEC1

    Plasmid
    #229536
    Purpose
    pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    Cas3
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229536-plasmid-map-sequence-id-458911

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  20. pCas3-CDA1

    Plasmid
    #229534
    Purpose
    pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    Cas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229534-plasmid-map-sequence-id-458891

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Showing: 7901 - 7920 of 8705 results