We narrowed to 16,384 results for: Jun

1. plentiCRISPR-v2 Puro KIAA0141/DELE1_sg1

   Plasmid

   #218524

   Purpose

   sgRNA targeting human KIAA0141/DELE1

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   DELE1 (DELE1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. plentiCRISPR-v2 Puro KIAA0141/DELE1_sg2

   Plasmid

   #218525

   Purpose

   sgRNA targeting human KIAA0141/DELE1

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   DELE1 (DELE1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-nEF-NpHR3.3-EYFP

   Plasmid

   #137151

   Purpose

   Viral expression of NpHR3.3-EYFP

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   NpHR3.3-EYFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   W179F

   Promoter

   nEF

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. LSB-hsa-miR-29a-3p

   Plasmid

   #103391

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-29a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-29a-3p target (MIR29A Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-SFFV-FLAG-TurboID-mutKIR-SQSTM1

   Plasmid

   #223719

   Purpose

   Expresses FLAG-TurboID-mutKIR-SQSTM1

   Depositor

   Benjamin Wolozin

   Article

   Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

   Insert

   SQSTM1 (SQSTM1 Human)
   

   Use

   Lentiviral

   Tags

   TurboID

   Expression

   Mammalian

   Mutation

   Mutation 347-DPSTGE-352 to 347-APSAAA-352

   Promoter

   SFFV

   Available since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pHR-SFFV-FLAG-TurboID-dPB1-SQSTM1

   Plasmid

   #223716

   Purpose

   Expresses FLAG-TurboID-dPB1-SQSTM1

   Depositor

   Benjamin Wolozin

   Article

   Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

   Insert

   SQSTM1 (SQSTM1 Human)
   

   Use

   Lentiviral

   Tags

   TurboID

   Expression

   Mammalian

   Mutation

   deletion 1-119

   Promoter

   SFFV

   Available since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pHR-SFFV-FLAG-TurboID-dUBA-SQSTM1

   Plasmid

   #223717

   Purpose

   Expresses FLAG-TurboID-dUBA-SQSTM1

   Depositor

   Benjamin Wolozin

   Article

   Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

   Insert

   SQSTM1 (SQSTM1 Human)
   

   Use

   Lentiviral

   Tags

   TurboID

   Expression

   Mammalian

   Mutation

   deletion 384-Stop codon

   Promoter

   SFFV

   Available since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pHR-SFFV-FLAG-TurboID-mutLIR-SQSTM1

   Plasmid

   #223718

   Purpose

   Expresses FLAG-TurboID-mutLIR-SQSTM1

   Depositor

   Benjamin Wolozin

   Article

   Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

   Insert

   SQSTM1 (SQSTM1 Human)
   

   Use

   Lentiviral

   Tags

   TurboID

   Expression

   Mammalian

   Mutation

   Mutation 335-DDDW-338 to 335-AAAA-338

   Promoter

   SFFV

   Available since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGEX Slp76-SH2

   Plasmid

   #46503

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Slp76 (LCP2 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 417-533

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRepair-mScI-CTNNB1

    Plasmid

    #153431

    Purpose

    Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)
    

    Use

    CRISPR; Hdr donor template

    Tags

    mScarlet-i

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1

    Plasmid

    #123194

    Purpose

    binary plant vector for transient dual luciferase (rLUC & fLUC with introns) expression

    Depositor

    Carolyn Lee-Parsons, Erin Cram

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    pNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LSB-hsa-miR-195-3p

    Plasmid

    #103311

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-195-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-195-3p target (MIR195 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV GFAP jGCaMP7b

    Plasmid

    #171118

    Purpose

    Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter

    Depositor

    Thomas Oertner

    Article

    Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

    Insert

    jGCaMP7b
    

    Use

    AAV

    Tags

    6 His Tag, T7 Tag, and Xpress Tag

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV-SI-112

    Plasmid

    #131127

    Purpose

    C-to-T base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Mutant EGFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-ACBEmax

    Plasmid

    #139106

    Purpose

    Mammalian ACBEmax expression vector (pSI-632)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDonor-GDH

    Plasmid

    #140629

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    Mini-Tn6677, T7lac-GDH gene cassette
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBp-FGFR3c-R248C

    Plasmid

    #45712

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR3 IIIc (FGFR3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    R248C

    Promoter

    Available since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pGem-sHyper:sAPX

    Plasmid

    #84738

    Purpose

    Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Inserts

    sAPX (SAPX Mustard Weed)
    sHyper (HyPer2 targeted to nucleus)
    

    Use

    Tags

    nuclear localisation signals from the coding sequ…

    Expression

    Plant

    Mutation

    Promoter

    pFMV/p35S

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBabe_puro_DEST_Flag_SKIL

    Plasmid

    #45265

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    SNON (SKIL Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

20. Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

    Plasmid

    #157986

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge