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CROP_C9_Puro_TOP1

Plasmid

#183323

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP1

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PDGFRA

Plasmid

#183313

Purpose

All-in-One CRISPRko system with a guide RNA that targets PDGFRA gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PDGFRA

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PARP1

Plasmid

#183312

Purpose

All-in-One CRISPRko system with a guide RNA that targets PARP1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PARP1

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_IDH1

Plasmid

#183300

Purpose

All-in-One CRISPRko system with a guide RNA that targets IDH1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

IDH1

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_HDAC2

Plasmid

#183298

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC2

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pV1093:SpRY

Plasmid

#172188

Purpose

Genome editing of C. albicans

Depositor

Doug Bernstein

Article

Evans et al mSphere. 2021 May 19;6(3). pii: 6/3/e003

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pL_FR_Hygro

Plasmid

#127171

Purpose

Frameshift reporter (control plasmid for screening)

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

Frameshift Reporter

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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JB82

Plasmid

#128911

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab10TN-BirA*-HA-MAO (RAB10 Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB81

Plasmid

#128910

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab10QL-BirA*-HA-MAO (RAB10 Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGDP3 vatD

Plasmid

#112885

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

vatD

Expression

Bacterial

Promoter

Pbla

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2f

Plasmid

#105065

Purpose

A yellow fluorescent Ca2+ indicator variant with low binding affinity and fast kinetics for expression in bacterial

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2f

Tags

6x His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT16

Plasmid

#89659

Purpose

expression of doubleTAL staple protein dT16 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT16

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT17

Plasmid

#89660

Purpose

expression of doubleTAL staple protein dT17 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT17

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT21

Plasmid

#89662

Purpose

expression of doubleTAL staple protein dT21 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT21

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT32

Plasmid

#89667

Purpose

expression of doubleTAL staple protein dT32 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT32

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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UAS-<FRT.STOP<Bxb1

Plasmid

#87643

Purpose

For creating drosophila transgenics expressing UAS-<FRT.STOP<Bxb1

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Bxb1

Expression

Insect

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB2795

Plasmid

#63650

Purpose

pCfB2795 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2802

Plasmid

#63655

Purpose

pCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2800

Plasmid

#63657

Purpose

pCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMV-LEU2

Plasmid

#65334

Purpose

standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

LEU2

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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