We narrowed to 13,275 results for: sequence
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Plasmid#46318DepositorInsert5 copies of A9 sequence from Ihh promoter
TagsluciferaseExpressionMammalianPromotermouse osteocalcin gene 2 promoterAvailable SinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shCUX1-5328
Plasmid#100815PurposeLentiviral vector expressing a doxycycline-inducible short hairpin RNA against CUX1 sequence starting at 5328 of M74099DepositorInsertTGCTGTTGACAGTGAGCGTCTTCTCGTTTGAAACTTTGAATAGTGAAGCCA
UseLentiviralExpressionMammalianAvailable SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/UAA-mEGFP-IRES-mCherry
Plasmid#49225PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterViral LTR (or CMV)Available SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-BCA-P(11)4
Plasmid#185780PurposeEncodes enzyme bovine carbonic anhydrase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-P(11)4
ExpressionBacterialPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmalc4x_E323K_TEV
Plasmid#47699PurposeExpresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323KDepositorInsertMaltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
TagsMBPExpressionBacterialMutationchanged glutamic acid 323 to lysinePromoterPtacAvailable SinceSept. 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
miR-144 Trap
Plasmid#60934Purposeto knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoterDepositorInsertmiR-144 Trap
UseLentiviralTagsGFPPromoterbidirectional phosphoglycerate kinase promoter (b…Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCA-ATG-intron-tdT3Myc
Plasmid#36883DepositorInserttdTomato-3Myc
Tags3 Myc tagsExpressionMammalianMutationbeta-globin intron containing a loxP site inserte…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWPT-ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49229PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_003
Plasmid#123817PurposeEncodes Nuclear import sequence as a Type 4a part to be used in the MTK systemDepositorInsertNLS
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-6
Plasmid#163132PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTH353-SUP45wt
Plasmid#29371DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
ExpressionYeastMutationWild type gene including genomic sequence 1432 bp…Available SinceMay 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-T2-NLuc
Plasmid#135938PurposeExpresses NanoLuciferase targeted to the mitochondira in mammalian cells.DepositorInsertNanoLuciferase
TagsT2 mitochondrial localization sequenceExpressionMammalianPromoterEF1aAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC
Plasmid#140200PurposeCloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsdLbCas12a crRNA scaffoldPromoterPgpdAAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-Cx35-Halo-C-in
Plasmid#71511PurposeMammalian expression plasmid encoding perch Cx35 with an internal HaloTag between amino acids 285 and 286 in the C-terminus.DepositorInsertConnexin 35
TagsHaloTag (internal to sequence)ExpressionMammalianAvailable SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-GPI-pA
Plasmid#109490PurposeMuscle specific zebrafish expression of mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1DepositorInsertactc1b-mKate2-GPI-pA
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Kp-2
Plasmid#163144PurposepLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastPromoterAOX1Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
HOXD12 (murine) pRSETa
Plasmid#8554DepositorInsertHOXD12 (Hoxd12 Mouse)
TagsHIS and XPressExpressionBacterialMutationFusion sequence is: ggatccATGAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49218PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterViral LTR (or CMV)Available SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
MTK4b_004
Plasmid#123843PurposeEncodes spacer sequence as a Type 4b part to be used in the MTK systemDepositorInsertSpacer
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmLSm4_A
Plasmid#145883PurposeInsect Expression of DmLSm4DepositorInsertDmLSm4 (LSm-4 Fly)
TagsV5-6xHisExpressionInsectMutationtwo silent mutations compared to the sequence of …Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only