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5K29 (PP-BRD3)

Plasmid

#101883

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

PP-BRD3

Use

Tags

6xHis, TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 30, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pTMiniTrex-mCherry-Shark2

Plasmid

#233144

Purpose

Serves as a template plasmid for amplifying Shark2 (Tet-ON) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark2

Use

Prokaryotic expression

Tags

3x Ty tag

Expression

Mutation

Codon optimized for Trypanosoma cruzi

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTMiniTrex-mCherry-Shark1

Plasmid

#233143

Purpose

Serves as a template plasmid for amplifying Shark1 (Tet-OFF) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark1

Use

Prokaryotic expression

Tags

3x Ty tag

Expression

Mutation

Codon optimized for Trypanosoma cruzi and changed…

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTMiniTrex-mCherry-Shark3

Plasmid

#233145

Purpose

Serves as a template plasmid for amplifying Shark3 (Theo-OFF) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark3

Use

Prokaryotic expression

Tags

3x Ty tag

Expression

Mutation

Codon optimized for Trypanosoma cruzi and changed…

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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BLA/Xpress

Plasmid

#1804

Depositor

Christian Tschudi

Article

Shen et al Mol Biochem Parasitol 2001 Mar;113(1):17

Insert

BLA/Xpress

Use

Tags

Xpress

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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pDONR221-Phelo

Plasmid

#48995

Purpose

Gateway adapted transfer of Phleomycin resistance gene

Depositor

Rick Tarleton

Article

Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

Insert

Sh ble

Use

Gateway attb1 & attb2

Tags

Expression

Mutation

Promoter

Available since

April 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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BLA/V5

Plasmid

#1806

Depositor

Christian Tschudi

Article

Shen et al Mol Biochem Parasitol 2001 Mar;113(1):17

Insert

BLA/V5

Use

Tags

V5

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2005

Availability

Academic Institutions and Nonprofits only
Phleo/Xpress

Plasmid

#1805

Depositor

Christian Tschudi

Article

Shen et al Mol Biochem Parasitol 2001 Mar;113(1):17

Insert

Phleo/Xpress

Use

Tags

Xpress

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2005

Availability

Academic Institutions and Nonprofits only
BLA/BB2

Plasmid

#1807

Depositor

Christian Tschudi

Article

Shen et al Mol Biochem Parasitol 2001 Mar;113(1):17

Insert

BLA/BB2

Use

Tags

BB2

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2005

Availability

Academic Institutions and Nonprofits only
Phleo/BB2

Plasmid

#1808

Depositor

Christian Tschudi

Article

Shen et al Mol Biochem Parasitol 2001 Mar;113(1):17

Insert

Phleo/BB2

Use

Tags

BB2

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2005

Availability

Academic Institutions and Nonprofits only