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H6GB1-CREB(1-280) C90A

Plasmid

#217982

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

C90A

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(160-280)

Plasmid

#217980

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-Creb5(T59/T61A)(HA)

Plasmid

#195025

Purpose

Gateway vector containing HA-tagged Creb5 with T59/T61A mutation

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with with T59/T61A mutation) (CREB5 Bovine)

Use

Gateway cloning

Tags

3xHA

Expression

Mutation

T59/T61A

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-Creb5(T59/T61D)(HA)

Plasmid

#195026

Purpose

Gateway vector containing HA-tagged Creb5 with T59/T61D mutation

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)

Use

Gateway cloning

Tags

3xHA

Expression

Mutation

T59/T61D

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer20-iCreb5(T59/T61A)(HA)

Plasmid

#195027

Purpose

HA-tagged Creb5(T59/T61A) in pInducer20 lentivirus destination vector

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with T59/T61A mutation) (CREB5 Bovine)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T59/T61A

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer20-iCreb5(T59/T61D)(HA)

Plasmid

#195028

Purpose

HA-tagged Creb5(T59/T61D) in pInducer20 lentivirus destination vector

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T59/T61D

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-T2A-ACREB-WPRE

Plasmid

#40867

Depositor

Harold Gainer

Article

Lubelski et al Brain Res. 2012 Jan 6;1429:18-28. Epub 2

Insert

ACREB

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) C90A, T172C

Plasmid

#217983

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

C90A, T172C

Promoter

T7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) A50C,C90A

Plasmid

#217985

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

A50C, C90A

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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GAL4-Creb5 (1- 128) T59/T61 A

Plasmid

#195032

Purpose

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with T59/T61 A mutation)

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with with T59/T61 A mutation) (CREB5 Bovine)

Use

Tags

Expression

Mammalian

Mutation

T59/T61 A

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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GAL4-Creb5 (1- 128) C18/C23 S

Plasmid

#195033

Purpose

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation)

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation) (CREB5 Bovine)

Use

Tags

Expression

Mammalian

Mutation

C18/C23 S

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEF086 MBP-CREB 127-135-His

Plasmid

#154075

Purpose

Expresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) T2C, C90A, T198C

Plasmid

#220235

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

T2C, C90A, T198C

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) C90A, T172C, T259C

Plasmid

#220234

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

C90A, T172C, T259C

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-mGFP-MutCREB(S133A)

Plasmid

#194642

Purpose

Expresses the fusion of monomeric-EGFP-tagged mutant CREB(S133A) in a Cre-dependent manner

Depositor

Jun-Hyeong Cho

Article

Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

Insert

CREB (Creb1 Rat)

Use

AAV

Tags

mEGFP

Expression

Mutation

S133A

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

Plasmid

#113692

Purpose

U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 (NEWENTRY )
SaCas9 gRNA Cassette (NEWENTRY )

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

NLS

Expression

Mutation

Promoter

Cytomegalo Virus(CMV)

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pJEP320-pAAV-U6SaCas9gRNA(CREB3)_EFS-GFP-KASH-pA

Plasmid

#113697

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Expression

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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HJP-207-IVS-Syn21-gp41-1C-CreBC-WPREUw

Plasmid

#162479

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreBC

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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HJP-177-IVS-Syn21-gp41-1C-CreBC-WPREw

Plasmid

#162474

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreBC

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter CREB site mutant pGL3Basic

Plasmid

#32732

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 Promoter (CCND1 Human)

Use

Luciferase

Tags

Expression

Mutation

-51 CREB site TAACGTCA deletion

Promoter

-962 CCND1 Promoter

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only