Addgene

Showing: 61 - 80 of 149 results

Results per page:

Sort results by:

pVF42

Plasmid

#229467

Purpose

Expresses RssB with S29C & E229C mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (S29C, E229C)

Use

Tags

Expression

Bacterial

Mutation

S29C, E229C

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pVF16

Plasmid

#229465

Purpose

Expresses RssB with D58P mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D58P)

Use

Tags

Expression

Bacterial

Mutation

D58P

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS26

Plasmid

#229471

Purpose

Expresses RssB with D104N mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D104N)

Use

Tags

10xHis

Expression

Bacterial

Mutation

D104N

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS23

Plasmid

#229470

Purpose

Expresses RssB with D104A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D104A)

Use

Tags

10xHis

Expression

Bacterial

Mutation

D104A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS11

Plasmid

#229464

Purpose

Expresses RssB with His Tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-Flex-TCB

Plasmid

#48332

Purpose

Cre-dependant TVA-mCherry fusion

Depositor

Liqun Luo

Article

Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

Insert

TVA receptor mCherry

Use

AAV

Tags

mCherry fusion to TVA receptor

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-FLEx(FRT)-TC

Plasmid

#67827

Purpose

Flp-dependent expression of TVA-mCherry fusion to mediate modifed rabies virus expression in cells

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

TVA-mCherry fusion

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-FLEx(FRT)-G

Plasmid

#67828

Purpose

Flp-dependent expression of rabies glycoprotein to mediate trans-synaptic spread of modifed rabies virus in cells

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

rabies glycoprotein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTCAV-FLEx(loxP)-FlpO

Plasmid

#67829

Purpose

Cre-dependent expression of FlpO from CAV2 virus

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

FlpO

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS19

Plasmid

#229475

Purpose

Expresses RssB with E135A, E136A, & E137A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Use

Tags

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS21

Plasmid

#229476

Purpose

Expresses RssB with E135A, E136A, & E137A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Use

Tags

10xHis

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTre2tet)::Fbxl21

Plasmid

#51890

Purpose

expression of Fbxl21

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Fbxl21 (Fbxl21 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV tet

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pAC-luc-TimRA

Plasmid

#33133

Depositor

Michael Rosbash

Article

Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. E

Insert

Tim-RA 3' UTR (tim Fly)

Use

Luciferase

Tags

Firefly Luciferase

Expression

Insect

Mutation

Promoter

Actin 5C

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pET29a-hNABP1-N

Plasmid

#128310

Purpose

Express hNABP1 N-terminus (1–141 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP1 N-terminal domain (OB fold, 1–141 aa) (NABP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

N-terminal domain (OB fold, 1–140 aa)

Promoter

T7

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV10-Flag-Fbxl21

Plasmid

#48752

Purpose

Expression of FLAG-tagged Fbxl21

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Fbxl21 (Fbxl21 Mouse)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pAG32-3HA-3FLAG

Plasmid

#154081

Purpose

For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

Depositor

Cornelia Kilchert

Article

Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

Insert

3xHA-3xFLAG-hygR

Use

Pcr-based tagging in yeast

Tags

3xHA-3xFLAG

Expression

Mutation

Promoter

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-Flex-TC66T

Plasmid

#48331

Purpose

Cre-dependant TVA receptor-mCherry fusion with a mutation (66T) in TVA

Depositor

Liqun Luo

Article

Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

Insert

TVA receptor mCherry fusion with the 66T mutation

Use

AAV and Cre/Lox

Tags

mCherry fusion to TVA receptor

Expression

Mammalian

Mutation

66T

Promoter

CAG

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-luc-CLK

Plasmid

#33132

Depositor

Michael Rosbash

Article

Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. E

Insert

CLK 3' UTR (Clk Fly)

Use

Luciferase

Tags

Firefly Luciferase

Expression

Insect

Mutation

Promoter

Actin 5C

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pfast bac-flag Fbxl3

Plasmid

#51894

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Fbxl3 (Fbxl21 Mouse)

Use

Tags

FLAG

Expression

Mutation

Promoter

polyhedrin promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
Danio alpha A-crystallin 1kb promoter/AcGFP

Plasmid

#98096

Purpose

aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)

Use

Gfp expressing

Tags

GFP

Expression

Mutation

Promoter

Danio alpha A-crystallin 1 kb fragment (-1028/-1)

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge