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pEB3-miRFP670nano

Plasmid

#127433

Purpose

EB3 (microtubule-associated protein) labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

EB3-miRFP670nano (MAPRE3 Human, Synthetic)

Use

Tags

miRFP670nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pmiRFP670nano3-NbGFP

Plasmid

#184686

Purpose

Nanobody to GFP labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3-NbGFP

Use

Tags

miRFP670nano3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pVimentin-miRFP670nano

Plasmid

#127436

Purpose

Vimentin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

Vimentin-miRFP670nano

Use

Tags

miRFP670nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGK:TRL-EFS:iRFP670

Plasmid

#68475

Purpose

Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

tTR-KRAB-2A-rtTA3-2A-Luciferase
iRFP670

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

EFS and pGK

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCytERM-emiRFP670-N1

Plasmid

#197180

Purpose

Expresses the fusion protein of CytERM-emiRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-emiRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pKeratin-miRFP670nano

Plasmid

#127437

Purpose

Keratin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

Keratin-miRFP670nano

Use

Tags

miRFP670nano

Expression

Mammalian

Mutation

Keratin V282L - please see depositor comments bel…

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMyosin-miRFP670nano

Plasmid

#127431

Purpose

LC-Myosin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano-LC-Myosin

Use

Tags

miRFP670nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pB2AR-miRFP670nano3

Plasmid

#184671

Purpose

Beta-2-adrenergic receptor internally tagged with miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

B2AR-miRFP670nano3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pActinin-miRFP670nano

Plasmid

#127432

Purpose

Alpha actinin labeling with near-infrared fluorescent protein

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

Alpha-actinin-miRFP670nano

Use

Tags

miRFP670nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-P1-N-miRFP670-C

Plasmid

#159438

Purpose

CRISPR knock-in donor construct with fluorescent reporter

Depositor

Jianhua Xing

Article

Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

Insert

miRFP670

Use

CRISPR

Tags

3' linker pair 1, 5' linker pair 1, C t…

Expression

Mutation

Promoter

CMV

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGas-miRFP670nano3

Plasmid

#184672

Purpose

G protein alpha-subunit internally tagged with miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

G protein alpha-s-miRFP670nano3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pB2AR-miRFP670nano

Plasmid

#127442

Purpose

Beta-2-adrenergic receptor internally tagged with miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

B2AR-miRFP670nano (ADRB2 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP670-2-N1

Plasmid

#197228

Purpose

Expresses the fusion protein of H2B-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

H2B-miRFP670-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg83-PDGFR_EF1a_iRFP670

Plasmid

#186311

Purpose

Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg83-PDGFR
iRFP670

Use

Tags

His, myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_P3-PDGFR_EF1a_iRFP670

Plasmid

#186305

Purpose

Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

P3-PDGFR
iRFP670

Use

Tags

HA, myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pDRF1 yemiRFP670

Plasmid

#224103

Purpose

Expressing yemiRFP670 in yeast

Depositor

Bas Teusink

Insert

yemiRFP670

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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P415 yemiRFP670

Plasmid

#224107

Purpose

Expressing yemiRFP670 in yeast

Depositor

Bas Teusink

Insert

yemiRFP670

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKeratin-miRFP670-2-N1

Plasmid

#197233

Purpose

Expresses the fusion protein of Keratin-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Keratin-miRFP670-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCx43-7-miRFP670-2-N1

Plasmid

#197223

Purpose

Expresses the fusion protein of Cx43-7-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-miRFP670-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCx43-7-iRFP670-N1

Plasmid

#197222

Purpose

Expresses the fusion protein of Cx43-7-iRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-iRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge