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Plasmid#66710PurposeExpresses AREL1 HECT domain in E. coliDepositorInsertKIAA0317 (AREL1 )
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
InsRA-TagBFP
Plasmid#79791PurposeInsulin receptor isoform A with a inter-domain TagBFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.DepositorInsertInsRA (INSR Human)
UseTagsTagBFP extracellularExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLEX_307-SOD1E133insTT
Plasmid#83446PurposeMammalian expression of SOD1E133insTT.DepositorInsertSOD1 (SOD1 Human)
UseLentiviralTagsExpressionMammalianMutationE133insTT (after GA)PromoterEF-1αAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EGFR_p.769_770insASV
Plasmid#82880PurposeGateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.DepositorInsertEGFR (EGFR Human)
UseGateway entry vectorTagsExpressionMutationASV insertion between AA 769 and 770PromoterNoneAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-5INS_16
Plasmid#102292PurposeYeast Surface Display of 5Ins_16 scFVDepositorInsertInsulin binding scFV
UseTags3x(G4S) Linker, G3S Linker, HA, and MycExpressionYeastMutationPromoterGAL1Available sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTD-C_sfYFPTwinStrep
Plasmid#45943Purposeexpresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion proteinDepositorInsertsynthetic sfYFP (codon usage adapted to P.putida KT2440)
UseTagsTwin-Strep-tagExpressionBacterialMutationPromoterlacIq/PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
RINS1(mut)
Plasmid#107291PurposePlasmid for the expression of the inactive mutant of RINS1 sensorDepositorInsertRINS1(mut)
UseTagssfGFPExpressionMammalianMutationtwo Arg/Ser mutations at the beginning and in the…PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
proinsulin-moxGFP2
Plasmid#218957PurposeExpresses proinsulin fused to moxGFP2 in bacteriaDepositorInsertproinsulin-moxGFP2
UseTagsproinsulinExpressionBacterialMutationPromoterT5Available sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
proinsulin-mGold
Plasmid#218953PurposeExpresses proinsulin fused to mGold in bacteriaDepositorInsertproinsulin-mGold
UseTagsproinsulinExpressionBacterialMutationPromoterT5Available sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EGFR_p.774_775insHV
Plasmid#82852PurposeGateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.DepositorInsertEGFR (EGFR Human)
UseGateway entry vectorTagsExpressionMutationHV insertion between AA 774 and 775PromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
natMX6-ins5
Plasmid#195042PurposepFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interferenceDepositorInsertNatR
UseYeast genomic targetingTagsS. cerevisiae DEG1 terminator, A. gossypii TEF pr…ExpressionMutationPromoterAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-5INS_EV3
Plasmid#102293PurposeYeast Surface Display of 5Ins_16_EV3DepositorInsertEvolved Insulin binding scFV
UseTags3x(G4S) Linker, G3S Linker, HA, and MycExpressionYeastMutationPromoterGAL1Available sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
InsRA-EBFP2
Plasmid#79797PurposeInsulin receptor isoform A with a inter-domain EBFP2 tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.DepositorInsertInsRA (INSR Human)
UseTagsEBFP2 extracellularExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
CAPZA1-TwinStrepII
Plasmid#83193PurposeExpression of tagged protein in mammalian cellsDepositorInsertCAPZA1 (Capza1 Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
GBSGFP insulator
Plasmid#121975PurposeGli activity reporter, mouse transgenesisDepositorInsertGBS(long)-GFP-insulator (Gnb5 Mouse)
UseMouse transgenesisTagsExpressionMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRIOBP-TwinStrepII
Plasmid#83201PurposeExpression of tagged protein in mammalian cellsDepositorInsertTRIOBP (Triobp Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
ins:MCS; cryaa:RFP
Plasmid#110282PurposeA zebrafish insulin promoter containing vector with multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.DepositorTypeEmpty backboneUseZebrafishTagsExpressionMutationPromoterinsulinAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKSII/INSeGFP
Plasmid#53787Purpose1 kb zebrafish insulin promoter driving eGFPDepositorInsertINS promoter/eGFP/pA
UseZebrafishTagseGFPExpressionBacterialMutationPromoterzebrafish insulin/ZIPAvailable sinceJan. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
TRP1MX6-ins4
Plasmid#195041PurposepFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interferenceDepositorInsertTRP1
UseYeast genomic targetingTagsS. cerevisiae DEG1 terminator, A. gossypii TEF pr…ExpressionMutationPromoterAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
kanMX6-ins6
Plasmid#195043PurposepFA6a derived selection cassette 5' flanked with tDEG1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interferenceDepositorInsertKanR
UseYeast genomic targetingTagsS. cerevisiae DEG1 terminator, A. gossypii TEF pr…ExpressionMutationPromoterAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only