We narrowed to 254 results for: petD;

1. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST

   Plasmid

   #223745

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   BCL2L13 soluble part (BCL2L13 Human)
   

   Use

   Tags

   EGFP-TEV-GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDUET-GST AO7RE ∆238-245

   Plasmid

   #139318

   Purpose

   GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   AO7 cloned into the first multiple cloning site o…

   Promoter

   T7 promoter-1

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETDuet1-LanCL2-H264A-C-Ter-His

   Plasmid

   #154187

   Purpose

   To express LanCL2-H264A in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL2
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   His264->Ala264

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)

   Plasmid

   #61941

   Purpose

   Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Inserts

   hUCH37 (ISF1) (UCHL5 Human)
   hNFRKB (1-117) (NFRKB Human)
   

   Use

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Construct ends at residue 117, stop codon introdu…

   Promoter

   T7

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

5. 213-petDuet1-GBD-LUMP--cdc42

   Plasmid

   #65973

   Purpose

   Dual expression of GBD-LUMP and cdc42

   Depositor

   Gerard Marriott

   Article

   Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

   Inserts

   LUMP
   cell division cycle 42 (CDC42 Human)
   

   Use

   Tags

   GBD (GTPase binding domain from ACK1; residues 44…

   Expression

   Bacterial

   Mutation

   constitutively active Cdc42 (Q61L)

   Promoter

   T7

   Available since

   July 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)

   Plasmid

   #182395

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusions

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-LZ-VenusN154
   VNp-LZ-VenusC155
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp) & Leucine Zi…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

   Plasmid

   #156463

   Purpose

   Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

   Depositor

   Andre Stiel

   Article

   Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

   Inserts

   Heme Oxygenase
   ReBphP-PCM
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETDuet1_6xHis-TEV-mCherry-WIPI2d (K87A/K88A)

   Plasmid

   #223751

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   6xHis-TEV-mCherry

   Expression

   Bacterial

   Mutation

   K87A/K88A

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETDUET-GST-AUP1 379-410:Ube2G2

   Plasmid

   #185335

   Purpose

   Used for co-expression of the AUP1 G2BR with UBE2G2 for crystallography

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 aa 379-410, Ube2G2 (AUP1 Mouse, Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETDuet1-LanCL2-C321A/C367A-C-Ter-His

    Plasmid

    #154188

    Purpose

    To express LanCL2-C321A/C367A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL2
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Cys321->Ala321; Cys367->Ala367

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGS

    Plasmid

    #61940

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-156) GSGS (NFRKB Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 156, residues 97-100 (N…

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pETDuet1_6xHis-TEV-mCherry-WIPI2d IDR (364-425aa)

    Plasmid

    #223790

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    WIPI2d IDR (WIPI2 Human)
    

    Use

    Tags

    6xHis-TEV-mCherry

    Expression

    Bacterial

    Mutation

    aa 364-425 only

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETDuet1_6xHis-TEV-mCherry-ATG13 IDR (191-517aa)

    Plasmid

    #223762

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    ATG13 IDR (ATG13 Human)
    

    Use

    Tags

    6xHis-TEV-mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETDuet1_NIX(1-182aa)-THROMBIN-GST (W36A/L39A; deltaLIR)

    Plasmid

    #223738

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    NIX (BNIP3L) soluble part (BNIP3L Human)
    

    Use

    Tags

    Thrombin cleavage-GST

    Expression

    Bacterial

    Mutation

    W36A/L39A; deletion of LIR

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (Y18A/L21A; deltaLIR)

    Plasmid

    #223739

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    FUNDC1 soluble part, mutant (FUNDC1 Human)
    

    Use

    Tags

    Thrombin cleavage-GST

    Expression

    Bacterial

    Mutation

    Y18A/L21A; deletion of LIR

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (W276A/I279A; deltaLIR1)

    Plasmid

    #223749

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BCL2L13 soluble part (BCL2L13 Human)
    

    Use

    Tags

    Thrombin cleavage-GST

    Expression

    Bacterial

    Mutation

    expresses aa 1-465, with W276A/I279A; deletion of…

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)

    Plasmid

    #223724

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    FIP200-CTR (RB1CC1 Human)
    

    Use

    Tags

    6xHis-TEV-EGFP

    Expression

    Bacterial

    Mutation

    aa 1429-1591 only

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (Y213A/I216A; deltaLIR2)

    Plasmid

    #223783

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BCL2L13 soluble part (BCL2L13 Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    Y213A/I216A; deletion of LIR2

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A; deltaLIR3)

    Plasmid

    #223775

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BCL2L13 soluble part (BCL2L13 Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    I224A/L227A; deletion of LIR3

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I307A/V310A; deltaLIR4)

    Plasmid

    #223776

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BCL2L13 soluble part (BCL2L13 Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    I307A/V310A; deletion of LIR4

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge