We narrowed to 161,641 results for: addgene

1. pZNF764.1.0-gDNA

   Plasmid

   #112394

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF764

   Depositor

   Kevin White

   Insert

   ZNF764 (ZNF764 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZNF57.1.0-gDNA

   Plasmid

   #112392

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF57

   Depositor

   Kevin White

   Insert

   ZNF57 (ZNF57 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZNF257.1.0-gDNA

   Plasmid

   #112390

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF257

   Depositor

   Kevin White

   Insert

   ZNF257 (ZNF257 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. STK10 (STK10A-c043)

   Plasmid

   #110255

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   STK10 (STK10 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 - lacO

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTHFR (MTHFRA-c042)

   Plasmid

   #110271

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MTHFR (MTHFR Human)
   

   Use

   Tags

   TEV-His10-Flag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUPD2b:TMV-3NTR

   Plasmid

   #106218

   Purpose

   Provides Nos terminator and 3' part of Tobacco mosaic virus (3NTR, ribozyme) as level 0 GoldenBraid part (GCTT-CGCT)

   Depositor

   Tomáš Moravec

   Insert

   Infectious clone of Tobacco mosaic virus, 3' part
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. 5J3U (TgPKA, 583.m05422)

   Plasmid

   #79525

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   583.m05422
   

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO

   Available since

   June 15, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. 5FEA (LdBRD19, LdBPK_363520.1)

   Plasmid

   #79520

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   LdBPK_363520.1
   

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO

   Available since

   June 15, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. 5CZG (Tb427.10.7420)

   Plasmid

   #79517

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   Tb427.10.7420
   

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO

   Available since

   June 15, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. 4Z31

    Plasmid

    #69958

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RC3H2:YTC020-E10:C234061 (RC3H2 Human)
    

    Use

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. 4Z0O

    Plasmid

    #69954

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ZCWPW2:JMC01B-H12:C203050 (ZCWPW2 Human)
    

    Use

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. 4YVD

    Plasmid

    #69960

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PLRG1:JMC102-C09:C233452 (PLRG1 Human)
    

    Use

    Baculovirus expression

    Tags

    His and TEV cleavage site

    Expression

    Mutation

    Promoter

    polyhedrin

    Available since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. 4QQM

    Plasmid

    #61950

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RFXANK:JMC029-A03:C213522 (RFXANK Human)
    

    Use

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. 4MYI

    Plasmid

    #60103

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PVX_084705:MAC04G-C11:C228845
    

    Use

    Baculovirus expression

    Tags

    6x His and TEV cleavage site

    Expression

    Mutation

    See comments

    Promoter

    polyhedrin

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pZC09 (pFA6a-TEV-6xGly-PrtA-His3MX)

    Plasmid

    #44073

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    6xGly linker, ADH1 transcription terminator, His3…

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. 4X PRE TK luc

    Plasmid

    #206159

    Purpose

    Luciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    4X PRE TK luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 12x PRE TK luc

    Plasmid

    #206163

    Purpose

    Luciferase reporter construct containing 12 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    12X PRE TK luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    min TK

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330_EZH2gRNA14

    Plasmid

    #199586

    Purpose

    Contains guide RNA to 5' end of mouse EZH2 gene

    Depositor

    Mauro Calabrese

    Article

    Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

    Insert

    EZH2 (Ezh2 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9(D10A)

    Plasmid

    #74495

    Purpose

    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

    Insert

    Cas9 D10A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    D10A

    Promoter

    pTeto

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-dCas13b-ADAR2DD

    Plasmid

    #190913

    Purpose

    Addgene #103871 with 3x FLAG on N-terminal of dCas13

    Depositor

    Timothy Jarome

    Article

    Farrell et al Mol Psychiatry. 2023 May 17. doi: 10.103

    Insert

    3X FLAG
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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