We narrowed to 18,370 results for: Met

1. pVC

   Plasmid

   #175063

   Purpose

   expresses mVenus and mCherry pair in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   mVenus + mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

   Promoter

   CMV Promoter

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ppst93-ANME2bHR1 MCR-NG

   Plasmid

   #192774

   Purpose

   Expresses NG-tagged ANME2bHR1 MCR in Methanococcus maripaludis

   Depositor

   William B. Whitman

   Article

   Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

   Insert

   Methyl coenzyme M reductase
   

   Use

   Synthetic Biology; Heterologous expression in met…

   Tags

   Flag-Strep2

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. Ppst93-ANME1G37 MCR-NG

   Plasmid

   #192773

   Purpose

   Expresses NG-tagged ANME1G37 MCR in Methanococcus maripaludis

   Depositor

   William B. Whitman

   Article

   Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

   Insert

   Methyl coenzyme M reductase
   

   Use

   Synthetic Biology; Heterologous expression in met…

   Tags

   Flag-Strep2

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. htpG2-GFP

   Plasmid

   #109388

   Purpose

   GFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Insert

   phtpG2-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PylT(UAG) PylS mCherry-P2A-eGFP(150TAG)

   Plasmid

   #174526

   Purpose

   Dual-fluorescence reporter for quantifying PylT(UAG) decoding efficiency

   Depositor

   Yu-Hsuan Tsai

   Article

   Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

   Insert

   PylS
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-104

   Plasmid

   #79842

   Purpose

   Expresses NLS-PpsR2-VP16 under CMV promoter

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

   Insert

   NLS-PpsR2-VP16
   

   Use

   Tags

   Nuclear localization signal of nuclear cap-bindin…

   Expression

   Mammalian

   Mutation

   RpPpsR2 cysteine 439 changed to serine

   Promoter

   CMV

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV MN (eGFP)

   Plasmid

   #89932

   Purpose

   Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

   Depositor

   Carl Novina

   Article

   Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

   Insert

   MN
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   M.SssI residues 2-272 fragment

   Promoter

   CMV

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CaYin1 NES

   Plasmid

   #33318

   Depositor

   Robert Campbell

   Article

   Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

   Insert

   CaYin1 NES
   

   Use

   Tags

   NES sequence LALKLAGLDIGS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ppst93-E50 MCR-NG

   Plasmid

   #192778

   Purpose

   Expresses NG-tagged E50 MCR in Methanococcus maripaludis

   Depositor

   William B. Whitman

   Article

   Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

   Insert

   Methyl coenzyme M reductase
   

   Use

   Synthetic Biology; Heterologous expression in met…

   Tags

   Flag-Strep2

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. Ppst93-ANME2cLM014 MCR-NG

    Plasmid

    #192777

    Purpose

    Expresses NG-tagged ANME2cLM014 MCR in Methanococcus maripaludis

    Depositor

    William B. Whitman

    Article

    Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

    Insert

    Methyl coenzyme M reductase
    

    Use

    Synthetic Biology; Heterologous expression in met…

    Tags

    Flag-Strep2

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. Ppst93-ANME2cE20 MCR-NG

    Plasmid

    #192775

    Purpose

    Expresses NG-tagged ANME2cE20 MCR in Methanococcus maripaludis

    Depositor

    William B. Whitman

    Article

    Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

    Insert

    Methyl coenzyme M reductase
    

    Use

    Synthetic Biology; Heterologous expression in met…

    Tags

    Flag-Strep2

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. Ppst93-ANME1BS MCR-NG

    Plasmid

    #192772

    Purpose

    Expresses NG-tagged ANME1BS MCR in Methanococcus maripaludis

    Depositor

    William B. Whitman

    Article

    Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

    Insert

    Methyl coenzyme M reductase
    

    Use

    Synthetic Biology; Heterologous expression in met…

    Tags

    Flag-Strep2

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. PylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

    Plasmid

    #174528

    Purpose

    Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

    Depositor

    Yu-Hsuan Tsai

    Article

    Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

    Insert

    PylS
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p415 Glo3 R59K S389,398 D

    Plasmid

    #112657

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 R59K S389,398D
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R59K, mutations (S389D, S398D) in Glo3 motif, pho…

    Promoter

    Met25

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p415 GFP-Glo3 S389,398 D

    Plasmid

    #112661

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    GFP-Glo3 S389,398D
    

    Use

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    mutations (S389D, S398D) in Glo3 motif, phosphomi…

    Promoter

    Met25

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PHBA03

    Plasmid

    #83537

    Purpose

    Constitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genes

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

    Insert

    pTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available Since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. RNA motif plasmid cloning backbone

    Plasmid

    #107253

    Purpose

    RNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kb

    Depositor

    Paul Khavari

    Article

    Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

    Insert

    RNA motif plasmid cloning backbone
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. hSTARR-seq_ORI vector

    Plasmid

    #99296

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. hSTARR-seq_SCP1 vector_blocking 4

    Plasmid

    #99319

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-GFP

    Plasmid

    #106281

    Purpose

    A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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