Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,651 results for: Tef

Clear keywords
Showing: 781 - 800 of 1651 results

  1. pαH-S-GSAS-2P-OMICRON

    Plasmid
    #180593
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180593-plasmid-map-sequence-id-352871

    Enlarge

  3. pαH-S-GSAS-OMICRON

    Plasmid
    #180423
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    180423-plasmid-map-sequence-id-354264

    Enlarge

  4. pαH-S-RRAR/XBB.1.5

    Plasmid
    #212993
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 with with furin side intact
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    Spike S-RRAR/XBB.1.5 (S )
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); furin site intake; (T19I…
    Promoter
    CMV
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212993-plasmid-map-sequence-id-423196

    Enlarge

  5. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213027
    Purpose
    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    D702Y (RT- reverse transcriptase catalytic dead)
    Promoter
    CMV
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213027-plasmid-map-sequence-id-452358

    Enlarge

  6. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213029
    Purpose
    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213029-plasmid-map-sequence-id-422050

    Enlarge

  7. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

    Enlarge

  13. pNIC28-BIRC7

    Plasmid
    #196363
    Purpose
    protein expression of the His-tagged full length protein
    Depositor
    Stefan Knapp
    Insert
    BIRC7/Livin
    Tags
    His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196363-plasmid-map-sequence-id-386127

    Enlarge

  14. pNIC28-BIRC3

    Plasmid
    #196366
    Purpose
    protein expression of the His-tagged full length protein
    Depositor
    Stefan Knapp
    Insert
    BIRC3/cIAP2
    Tags
    His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196366-plasmid-map-sequence-id-386071

    Enlarge

  15. pETTEV-SENP8

    Plasmid
    #196361
    Purpose
    protein expression of the His-tagged full length protein
    Depositor
    Stefan Knapp
    Insert
    SENP8/NEDP1
    Tags
    His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196361-plasmid-map-sequence-id-386147

    Enlarge

  16. pNIC28-SENP1

    Plasmid
    #196354
    Purpose
    protein expression of the His-tagged catalytic domain
    Depositor
    Stefan Knapp
    Insert
    SENP1 catalytic domain
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    contains only E419-L644
    Promoter
    T7
    Available Since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    196354-plasmid-map-sequence-id-386265

    Enlarge

  17. pNIC28-SENP2

    Plasmid
    #196355
    Purpose
    protein expression of the His-tagged catalytic domain
    Depositor
    Stefan Knapp
    Insert
    SENP2 catalytic domain
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    contains only D364-L589
    Promoter
    T7
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196355-plasmid-map-sequence-id-392708

    Enlarge

  18. pKM263-USP7

    Plasmid
    #196365
    Purpose
    protein expression of the GST-tagged catalytic domain
    Depositor
    Stefan Knapp
    Insert
    USP7 catalytic domain
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    contains only S207-E560
    Promoter
    T7
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196365-plasmid-map-sequence-id-386294

    Enlarge

  19. pGTVL2-SENP6

    Plasmid
    #196359
    Purpose
    protein expression of the GST-tagged catalytic domain
    Depositor
    Stefan Knapp
    Insert
    SENP6 catalytic domain
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    contains only E652-A1095
    Promoter
    T7
    Available Since
    April 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196359-plasmid-map-sequence-id-386314

    Enlarge

  20. pKM263-USP40

    Plasmid
    #196356
    Purpose
    protein expression of the GST-tagged catalytic domain
    Depositor
    Stefan Knapp
    Insert
    USP40 catalytic domain
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    contains only R35-Q383
    Promoter
    T7
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196356-plasmid-map-sequence-id-386259

    Enlarge

Showing: 781 - 800 of 1651 results