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pT7-EGFP-C1-HsTDRD3_K

Plasmid

#146768

Purpose

Mammalian Expression of HsTDRD3

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsTDRD3 (TDRD3 Human)

Use

Tags

Expression

Mammalian

Mutation

two non silent mutations H100R and P508H compared…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTDRD3-EBMmut_K

Plasmid

#146769

Purpose

Mammalian Expression of HsTDRD3-EBMmut

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsTDRD3-EBMmut (TDRD3 Human)

Use

Tags

Expression

Mammalian

Mutation

two non silent mutations H100R and P508H compare…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsSmg6_1-515_I

Plasmid

#146566

Purpose

Bacterial Expression of HsSmg6_1-515

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1-515 (SMG6 Human)

Use

Tags

Expression

Bacterial

Mutation

two silent mutations and two non silent mutation …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pKSB-AeU6-1gRNA

Plasmid

#183912

Purpose

gRNA-expressing plasmid under Aedes aegypti AAEL017702 U6 promoter

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

gRNA scaffold for protospacer cloning into BbsI sites

Use

Tags

Expression

Mutation

Promoter

Aedes aegypti U6 promoter (AAEL017702)

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pKSB-AeU6-2gRNA

Plasmid

#183913

Purpose

gRNA-expressing plasmid under Aedres aegypti AAEL017774 U6 promoter

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

gRNA scaffold for protospacer cloning into BbsI sites

Use

Tags

Expression

Mutation

Promoter

Aedes aegypti U6 promoter (AAEL017774)

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pKSB-AeU6-3gRNA

Plasmid

#183914

Purpose

gRNA-expressing plasmid under Aedres aegypti AAEL017763 U6 promoter

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

gRNA scaffold for protospacer cloning into BbsI sites

Use

Tags

Expression

Mutation

Promoter

Aedes aegypti U6 promoter (AAEL017763)

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsC2orf68-EBMmut_K

Plasmid

#146761

Purpose

Mammalian Expression of HsC2orf68-EBMmut

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsC2orf68-EBMmut (C2orf68 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-BTLA (PD-1 ITIM)-mGFP

Plasmid

#180818

Purpose

Expression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

BTLA (PD-1 ITIM) (BTLA Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mutation

replaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…

Promoter

SFFV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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OA-1010A

Plasmid

#176078

Purpose

pBac-10xUAS-L596_g9608-GGS4-HAx3-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

L596_g9608.t1

Use

Tags

3xHA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1010B

Plasmid

#176079

Purpose

pBac-10xUAS-L596_g25050-GGS4-HAx3-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

L596_g25050.t1

Use

Tags

3xHA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1010C

Plasmid

#176080

Purpose

pBac-10xUAS-Ce FAR-8-GGS4-HAx3-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

Ce FAR-8

Use

Tags

3xHA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1010D

Plasmid

#176081

Purpose

pBac-10xUAS-Anccey_04371-GGS4-HAx3-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

Ce FAR-8

Use

Tags

3xHA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE-bNup98-DN-NLS-mClover3

Plasmid

#171495

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged bovine Nup98 DN mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

Nup98-DN (NUP98 B. tarus (bovine))

Use

Pgehme

Tags

mClover3

Expression

Mutation

Promoter

T7

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19_4xT790_28bp

Plasmid

#114720

Purpose

4 x 28 bp repeats of the EGFR WT allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_T790_28bp

Plasmid

#114722

Purpose

28 bp fragment of the WT EGFR allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-hSOX10-G1238A-C1240T

Plasmid

#24739

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

G replaced with A at position 1238 and C replaced…

Promoter

Available since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pPICZ:hTRAAK(W262S)

Plasmid

#130670

Purpose

P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

KCNK4 (KCNK4 Human)

Use

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

N104Q, N108Q, W262S

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pIRES:hTRAAK(W262S)

Plasmid

#133078

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pIRES:hTRAAK(G124I)

Plasmid

#133079

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)

Use

Tags

Expression

Mammalian

Mutation

G124I

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pET28-SpRY-NLS-6xHis (gbr2101)

Plasmid

#181743

Purpose

bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Insert

bacterial codon optimized SpRY

Use

In vitro transcription; t7 promoter

Tags

BPNLS(SV40)-6xHis and MKIEE MBP tag

Expression

Bacterial

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

Promoter

T7

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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