We narrowed to 834 results for: chm

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pCellFree_G03 AKT1

Plasmid

#67102

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Publication

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

AKT1 (AKT1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 EHD2

Plasmid

#67107

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Publication

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

EHD2 (EHD2 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

S320T, E384D, T387A, H388Q, T405V, V407A, H418R, …

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-GFP-I3

Plasmid

#61401

Purpose

Expresses GFP-tagged Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Publication

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pCA528 IST1 L328D, L355A (1-366)

Plasmid

#193040

Purpose

Bacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.

Depositor

Wesley Sundquist

Publication

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

L328D, L355A

Promoter

T7

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1, residues 315-360, no Cys

Plasmid

#180643

Purpose

Bacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.

Depositor

Wesley Sundquist

Publication

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

residues 315-360

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-Exo-Blast-BFP

Plasmid

#196721

Purpose

Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.

Depositor

Bernhard Schmierer

Insert

Cas9-Exo1-T2A-BSD-TagBFP

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Promoter

EF1a

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry_C1 I3

Plasmid

#61398

Purpose

Expresses mCherry-tagged Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Publication

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pLJC5-HK2-3xFLAG

Plasmid

#239246

Purpose

HK2 lentiviral overexpression vector

Depositor

Jason Cantor

Publication

Huggler et al Nat Metab. 2026 Jan 16. doi: 10.1038/s42

Insert

HK2 (HK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert contains the set of silent mutations descr…

Promoter

Ubc

Available since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLJC6-HK2-3xFLAG

Plasmid

#239250

Purpose

HK2 lentiviral overexpression vector

Depositor

Jason Cantor

Publication

Huggler et al Nat Metab. 2026 Jan 16. doi: 10.1038/s42

Insert

HK2 (HK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert contains the set of silent mutations descr…

Promoter

Ubc

Available since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pDONR223 ATG3 C264A

Plasmid

#129293

Purpose

Gateway entry clone encoding human ATG3 C264A (catalytic inactive)

Depositor

Robin Ketteler

Publication

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway Cloning

Mutation

changed Cysteine 264 to Alanine

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 ATG3 K243R

Plasmid

#129294

Purpose

Gateway entry clone encoding human ATG3 K243R

Depositor

Robin Ketteler

Publication

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway Cloning

Mutation

changed Lysine 243 to Arginine

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-d80

Plasmid

#114030

Purpose

Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

Depositor

Geert Kops

Publication

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-d80-HEC1 (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 1 to 80 deleted

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-8A/S15D

Plasmid

#114031

Purpose

Inducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell lines

Depositor

Geert Kops

Publication

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1-8A/S15D (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 8A/S15D

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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18065-M02-412

Plasmid

#225665

Purpose

Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue

Depositor

Dominic Esposito

Publication

Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

Inserts

dsmCherry
delCD19 (CD19 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

C-terminally truncated (aa 1-333 only)

Promoter

SORE6-mCMVp and WPRE-SV40p

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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18065-M04-412

Plasmid

#225667

Purpose

Lentivirus protein expression. Control plasmid.

Depositor

Dominic Esposito

Publication

Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

Inserts

dsmCherry
delCD19 (CD19 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

C-terminally truncated (aa 1-333 only)

Promoter

WPRE-SV40p and mCMVp

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1, residues 316-366, C-Cys

Plasmid

#191234

Purpose

Bacterial expression for IST1 residues 316-366, contains non-native C-terminal Cysteine for labeling

Depositor

Wesley Sundquist

Publication

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 316-366, contains nonnative C-terminus f…

Promoter

T7

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223 ATG3 allKR

Plasmid

#129295

Purpose

Gateway entry clone encoding human ATG3 with all 22 lysine residues mutated to arginine

Depositor

Robin Ketteler

Publication

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway Cloning

Mutation

changed all 22 Lysine residues to Arginine

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-GFP-I3(V41A/W43A)

Plasmid

#61402

Purpose

Expresses GFP-tagged, PP1 binding-deficient Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Publication

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

inhibitor-3 (PPP1R11 Human)

Tags

GFP

Expression

Mammalian

Mutation

V41A/W43A

Promoter

CMV

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pLAP-MIS12-KARD-3D

Plasmid

#114058

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Publication

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD-3D (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

BUBR1-S670D, S676D and T680D

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLAP-MIS12-KARD-3A

Plasmid

#114059

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Publication

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD-3A (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

BUBR1-S670A, S676A and T680A

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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