We narrowed to 8,526 results for: construct
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Plasmid#72459PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Thr/Trp165>Phe mutant (AaPurE1fwf-S57T)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
ExpressionBacterialMutationMutation changes tryptophan-34 to phenylalanine, …PromoterT7Available SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK565
Plasmid#72454PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Ala/Trp165>Phe mutant (AaPurE1fwf-H59A)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
ExpressionBacterialMutationMutation changes tryptophan-34 to phenylalanine, …PromoterT7Available SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK578
Plasmid#72457PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Ala/Trp165>Phe mutant (AaPurE1fwf-S57A)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
ExpressionBacterialMutationMutation changes tryptophan-34 to phenylalanine, …PromoterT7Available SinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK569
Plasmid#72455PurposeProduces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic geneDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
TagsHis6 and TEV cleavage siteExpressionBacterialMutationsynthetic gene with ~51 silent substitutionsPromoterT7Available SinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK585
Plasmid#72458PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Cys/Trp165>Phe mutant (AaPurE1fwf-S57C)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
ExpressionBacterialMutationMutation changes tryptophan-34 to phenylalanine, …PromoterT7Available SinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTF257 (pFA6a-TEV-6xGly-His3MX)
Plasmid#44068DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…Available SinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF258 (pFA6a-PSc-6xGly-His3MX)
Plasmid#44062DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…Available SinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF261 (pFA6a-TEV-6xGly-HphMX)
Plasmid#44080DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…Available SinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF262 (pFA6a-PSc-6xGly-HphMX)
Plasmid#44074DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…Available SinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF259 (pFA6a-TEV-6xGly-KanMX)
Plasmid#44092DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…Available SinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
RGB-S reporter
Plasmid#207841PurposeA three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coliDepositorInsertsRpoH sensing construct
SOS sensing construct
RpoS sensing construct
ExpressionBacterialPromoterPosmY, PsulA, and grpEAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P1-LoxP-Neo-N-miRFP670-C
Plasmid#159434PurposeCRISPR knock-in donor construct with fluorescent reporter and removable selectable markerDepositorInsertsNeomycin
miRFP670
UseCRISPRTags3' linker pair 1, 5' linker pair 1, C t…PromoterSV40Available SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-GEM-GECO1
Plasmid#32463DepositorInsertGEM-GECO1
UseLab constructedTags6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…ExpressionBacterialMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…Available SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
TOPO UBC
Plasmid#68456PurposeTOPO construct expressing UBC for generation of GMAP-compatible promoterDepositorAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-GEX-GECO1
Plasmid#32464DepositorInsertGEX-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…Available SinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
HCKan-P-TEF2
Plasmid#65337Purposeconstruction of TEF2 promoter in HCKan-P receiving vectorDepositorInsertTEF2 promoter
UseSynthetic BiologyAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
HCKan-T-TEF2
Plasmid#65340Purposeconstruction of TEF2 terminator in HCKan-P receiving vectorDepositorInsertTEF2 terminator
UseSynthetic BiologyAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
HCKan-P-TDH3
Plasmid#65336Purposeconstruction of TDH3 promoter in HCKan-P receiving vectorDepositorInsertTDH3 promoter
UseSynthetic BiologyAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
HCKan-T-ADH1
Plasmid#65339Purposeconstruction of ADH1 terminator in HCKan-P receiving vectorDepositorInsertADH1 terminator
UseSynthetic BiologyAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
HCKan-P-CYC1
Plasmid#65338Purposeconstruction of CYC1 promoter in HCKan-P receiving vectorDepositorInsertCYC1 promoter
UseSynthetic BiologyAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only