We narrowed to 18,937 results for: Tec

1. pcDNA-Flag-LmoCas7

   Plasmid

   #126483

   Purpose

   Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas7
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Flag-LmoCas5

   Plasmid

   #126484

   Purpose

   Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas5
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 GW-mCit-PA

   Plasmid

   #113449

   Purpose

   ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Gateway shuttle vector

   Tags

   mCitrine-ProteinA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. B*58:02:01

   Plasmid

   #203262

   Purpose

   Overexpress HLA allele

   Depositor

   Derin Keskin, Catherine Wu

   Article

   Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

   Insert

   B*58:02:01 (HLA-B Human)
   

   Expression

   Mammalian

   Available Since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA_B2AR-q-pep

   Plasmid

   #47437

   Purpose

   FRET sensor for beta2-adrenergic receptor

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

   Insert

   beta2-adrenergic receptor (ADRB2 Human)
   

   Tags

   10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pVC297 VEGF Site#1

   Plasmid

   #47505

   Purpose

   human gRNA expression vector targeting VEGF

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-VEGF site 1 (VEGFA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRIM27 gRNA (BRDN0001145893)

   Plasmid

   #77516

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM27

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM27 (TRIM27 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. ROCK2 gRNA (BRDN0001147800)

   Plasmid

   #76349

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ROCK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ROCK2 (ROCK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-mA1-BE3

   Plasmid

   #113421

   Purpose

   Expresses mA1-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   mA1-BE3 (Apobec1 S. pyogenes and Bacteriophage PBS2, Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ265G2

    Plasmid

    #164723

    Purpose

    Gateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A(Y130F)-ziSpyMac(D10A)-UGI)
    

    Use

    CRISPR; Gateway compatible a3a/y130f-ispymaccas9-…

    Expression

    Plant

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVC228 VEGF Site#3

    Plasmid

    #47507

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 3 (VEGFA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-BE3RA-P2A-Puro

    Plasmid

    #110839

    Purpose

    Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Mutation

    D10A

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. SEPHS2 gRNA (BRDN0001148921)

    Plasmid

    #77661

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SEPHS2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SEPHS2 (SEPHS2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. AA290

    Plasmid

    #215949

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD55_v4; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AA286

    Plasmid

    #215948

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD47_v6; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CDK2-3XFlag-pBabe

    Plasmid

    #73557

    Purpose

    vector encoding a fusion protein between CDK2 and 3 Flag tags

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    CDK2 (CDK2 Human)
    

    Use

    Retroviral

    Tags

    3X-FLAG

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

17. VRQR-HF1 variant

    Plasmid

    #138563

    Purpose

    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR-HF1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_UdgX-EE-UdgX-nCas9-RBMX

    Plasmid

    #163565

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-nCas9-RBMX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pv9_CBX7_2xChromo-13XL-BASU

    Plasmid

    #179413

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.2xChromo-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7.2xChromo-13XL-BASU
    

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30a-SARS-CoV2-ORF3b (S23Q)

    Plasmid

    #165088

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
    

    Expression

    Bacterial

    Mutation

    Stop codon to glutamine at 23rd amino acid

    Available Since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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