We narrowed to 24,924 results for: crispr

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pCfB2909(XII-5 MarkerFree)

Plasmid

#73281

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSEED

Plasmid

#219658

Purpose

Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)

Depositor

Markus Affolter

Publication

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Empty SEED Cassette (3xP3-dsRed)

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSH424-ssr

Plasmid

#198026

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Publication

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Sm resistance cassette

Expression

Bacterial

Promoter

SmR promoter

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pH-mono-MAD7

Plasmid

#170134

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Publication

Lin et al J Genet Genomics. 2021 May 3. pii: S1673

Insert

MAD7(ErCas12a)

Use

CRISPR

Expression

Plant

Promoter

maize Ubiquitin-1, switchgrass Ubiquitin-1

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pX-EGFP-g1

Plasmid

#107273

Purpose

eGFP sgRNA-1 and Cas9 expression vector (aka. pX-ps1)

Depositor

Knut Woltjen

Publication

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP sgRNA-1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pUD628

Plasmid

#103018

Purpose

expression of a Cpf1 programming crRNA targetting ADE2 (crADE2-3.S)

Depositor

Jean-Marc Daran

Publication

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crADE2-3.S (ADE2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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AY22_pgRNA.R5.1

Plasmid

#100294

Purpose

CCR5-targeting gRNA expression plasmid

Depositor

Manuel Goncalves

Publication

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA spacer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6 RNA Pol III promoter human snRNA

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAct:dCas9-VP64

Plasmid

#78901

Purpose

Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pR26-CMVconst

Plasmid

#127373

Purpose

Allows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

Depositor

Lance Miller

Publication

Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Promoter

CMV enhancer/promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCas9-HE-Geminin

Plasmid

#109402

Purpose

Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron

Depositor

Jean-Paul Concordet

Publication

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HE-Geminin

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-TRE3G-BE3RA-PGK-Puro

Plasmid

#110846

Purpose

Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Mutation

D10A

Promoter

TRE3G

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV_Actb HR donor

Plasmid

#97317

Purpose

HR donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Publication

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HR donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pX330-PITCh-COX8A

Plasmid

#227308

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of COX8A for knock-in.

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA Targeting C-terminus of COX8A (COX8A Human)

Use

CRISPR

Expression

Mammalian

Promoter

U67

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-CANX

Plasmid

#227279

Purpose

Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA Targeting C-terminus of CANX (CANX Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-LacI-GFP

Plasmid

#220198

Purpose

mini-Tn7 GFP expression vector - LacI/pLac promoter

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

LacI/pLac

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCH62 (denAsCas12a-KRAB piggyBac)

Plasmid

#217333

Purpose

piggyBac expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Publication

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

denAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Expression

Mammalian

Mutation

D908A/E174R/S542R/K548R

Promoter

CAG

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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EC67841

Plasmid

#209512

Purpose

Cloning in complete guide expression cassettes, with Cas12a (D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_0_mKate2_synCoTC

Plasmid

#199469

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mKate2

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSA_2_mKate2_synCoTC

Plasmid

#199471

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mKate2

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSA_2_mTagBFP2_synCoTC

Plasmid

#199474

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mTagBFP2

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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